Abstract
C-, Q-, H-, and Ag-banding have been carried out on the polytene chromosomes of Rhynchosciara hollaenderi. The results of these techniques are presented and are correlated with the molecular data which exists for Rhynchosciara polytene chromosomes. By systematic organization of both banding and molecular data, we have attempted to give as complete a picture as possible of the characteristics of the differentially banding regions. This presents an organized method of approaching mechanisms of banding in terms of structural and functional aspects of the intact chromosome. Polytene chromosomes are particularly suited for this type of analysis and with them, both developmental and evolutionary changes can be conveniently utilized for additional insights into the functions of banding regions.
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References
Arrighi, F.E., Saunders, G.F.: The relationship between repetious DNA and constitutive heterochromatin with special reference to man. In: Modern Aspects of Cytogenetics: Constitutive Heterochromatin in Man. Symp. Medica Hoechst 1973
Botchan, P., Reeder, R.H., Dawid, I.B.: Restriction analysis of the nontranscribed spacers of Xenopus laevis ribosomal DNA. Cell 11, 599–607 (1977)
Cervenka, J., Thorn, H.L., Gorlin, R.J.: Structural basis of banding pattern of human chromosomes. Cytogenet. Cell Genet. 12, 81–86 (1973)
Comings, D.E., Avelino, E.S., Okada, T.A., Wyant, H.E.: The mechanism of C- and G-banding of chromosomes. Exp. Cell Res. 77, 469–493 (1973)
Comings, D.E., Drets, M.E.: Mechanisms of chromosome banding. IX. Are variations in DNA base composition adequate to account for Quinacrine, Hoechst 33258, and Daunomycin banding? Chromosoma (Berl.) 65, 199–211 (1976)
Comings, D.E., Harris, D.C., Okada, T.A., Holmquist, G.: Nuclear proteins. IV. Deficiency of non-histone proteins in condensed chromatin of Drosophila virilis and mouse. Exp. Cell Res. 105, 349–365 (1977)
Comings, D.E., Kovacs. B.W., Avelino, E., Harris, D.C.: Mechanisms of chromosome banding. V. Quinacrine banding. Chromosoma (Berl.) 50, 111–145 (1975)
Denton, T.E., Howell, W.M., Barrett, J.V.: Human nucleolar organizer chromosomes; satellite associations. Chromosoma (Berl.) 55, 81–84 (1976)
Eckhart, R.A., Gall, J.G.: Satellite DNA associated with heterochromatin in Rhynchosciara. Chromosoma (Berl.) 32, 407–427 (1971)
Hennig, W., Meer, B.: Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature (Lond.) New Biol. 233, 70–72 (1971)
Holmquist, G.: Hoechst 33258 fluorescent staining of Drosophila chromosomes. Chromosoma (Berl.) 49, 333–356 (1975)
Howell, W.M.: Visualization of ribosomal gene activity: silver stains proteins associated with rRNA transcribed from oocyte chromosomes. Chromosoma (Berl.) 62, 361–367 (1977)
Howell, W.M., Denton, T.E.: Negative silver staining in A-T and satellite DNA-rich regions of human chromosomes. Chromosoma (Berl.) 57, 165–169 (1976)
Hsu, T.C., Arrighi, F.E.: Distribution of constitutive heterochromatin in mammalian chromosomes. Chromosoma (Berl.) 34, 243–253 (1971)
Hsu, T.C., Arrighi, F.E., Saunders, G.F.: Compositional heterogeneity of human heterochromatin. Proc. nat. Acad. Sci. (Wash.) 69, 1464–1466 (1972)
Jones, K.W., Bishop, J.D., Brito-da-Cunha, A.: Complex formation between poly-r (U) and various chromosome loci in Rhynchosciara. Chromosoma (Berl.) 43, 375–390 (1973)
Kongsuwan, K., Smyth, D.R.: Q-bands in Lilium and their relationship to C-banded heterochromatin. Chromosoma (Berl.) 60, 169–178 (1977)
Latt, S.A.: Microfluorometric detection of DNA replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)
MacKay, R.D.G.: The mechanisms of G- and C-banding in mammalian metaphase chromosomes. Chromosoma (Berl.) 44, 1–14 (1973)
Pardue, M.L., Gall, J.G.: Chromosomal localization of satellite DNA. Science 168, 1356–1358 (1970)
Pardue, M.L., Gerbi, S.A., Eckart, R.A., Gall, J.G.: Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma (Berl.) 29, 268–290 (1970)
Rudkin, G.T.: Non-replicating DNA in Drosophila. Genetics, Suppl., 61, 227–238 (1969)
Rudkin, G.T., Tartoff, K.: Repetitive DNA in polytene chromosomes of Drosophila melanogaster. Cold Spr. Harb. Symp. quant. Biol. 38, 397–403 (1973)
Scheres, J.M.J.C.: The effect of cations on C-band formation in human chromosomes. Cytogenet. Cell. Genet. 18, 2–12 (1977)
Stocker, A.J.: Correspondence of silver banding with rRNA hybridization sites in polytene chromosomes of Rhynchosciara hollaenderi. Exp. Cell Res. (in press, 1978)
Stocker, A.J., Pavan, C.: The influence of ecdysterone on gene amplification, DNA synthesis, and puff formation in the salivary gland chromosomes of Rhynchosciara hollaenderi. Chromosoma (Berl.) 45, 295–319 (1974)
Stocker, A.J., Pavan, C.: Developmental puffing patterns in salivary gland chromosomes of Rhynchosciara hollaenderi. Chromosoma (Berl.) 62, 17–47 (1977)
Sumner, T.A.: A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75, 305–306 (1972)
Tantravahi, R., Miller, D.A., Miller, O.J.: Ag-staining of nucleolus organizer regions of chromosomes after Q-, C-, G-, or R-banding procedures. Cytogenet. Cell Genet. 18, 364–369 (1977)
Varley, J.: Patterns of silver staining of human chromosomes. Chromosoma (Berl.) 61, 207–214 (1977)
Weisblum, B., de Haseth, P.L.: Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylate-rich regions in DNA. Proc. nat. Acad. Sci. (Wash.) 69, 629–632 (1972)
Woodcock, D.M., Sibatani, A.: Differential variations in the DNA of Drosophila melanogaster during development. Chromosoma (Berl.) 50, 147–173 (1975)
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Stocker, A.J., Fresquez, C. & Lentzios, G. Banding studies on the polytene chromosomes of Rhynchosciara hollaenderi . Chromosoma 68, 337–356 (1978). https://doi.org/10.1007/BF00327169
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DOI: https://doi.org/10.1007/BF00327169