Abstract
Chromosomes of Chinese hamster strain cells were air-dried on slides after BrdU substitution for two or three rounds of replication. The preparations were treated with 20% PCA at 55° C for 20–30 min, or 5N HCl at 55° C for 15–20 min. After staining with Giemsa, unifilarly BrdU-substituted chromatids stained faintly and bifilarly substituted chromatids stained darkly. Such a pattern of sister chromatid differential staining was confirmed by the examination of metaphase cells grown with BrdU for three rounds of replication.
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Takayama, S., Sakanishi, S. Differential giemsa staining of sister chromatids after extraction with acids. Chromosoma 64, 109–115 (1977). https://doi.org/10.1007/BF00327050
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DOI: https://doi.org/10.1007/BF00327050