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Expression of the Aspergillus niger glucose oxidase gene in A. niger, A. nidulans and Saccharomyces cerevisiae

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Summary

We report the cloning of the Aspergillus niger glucose oxidase gene and its use to elevate glucose oxidase productivity in A. niger by increasing the gene dosage. In addition, the gene has been introduced into A. nidulans where it provides the novel capacity to produce glucose oxidase. A plasmid, in which DNA encoding the mature form of glucose oxidase was preceded by a Saccharomyces cerevisiae secretion signal, effected high-level production of extracellular glucose oxidase in this yeast.

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Authors and Affiliations

  1. Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK

    H. Whittington, S. Kerry-Williams, K. Bidgood, N. Dodsworth, J. Peberdy & M. Dobson

  2. Delta Biotechnology Ltd., Castle Court, Castle Boulevard, NG7 1FD, Nottingham, UK

    H. Whittington, E. Hinchliffe & D. J. Ballance

Authors
  1. H. Whittington
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  2. S. Kerry-Williams
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  3. K. Bidgood
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  4. N. Dodsworth
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  5. J. Peberdy
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  6. M. Dobson
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  7. E. Hinchliffe
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  8. D. J. Ballance
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Communicated by B.S. Cox

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Whittington, H., Kerry-Williams, S., Bidgood, K. et al. Expression of the Aspergillus niger glucose oxidase gene in A. niger, A. nidulans and Saccharomyces cerevisiae . Curr Genet 18, 531–536 (1990). https://doi.org/10.1007/BF00327024

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  • DOI: https://doi.org/10.1007/BF00327024

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