Summary
Histochemical data are presented concerning distributions of succinic dehydrogenase (SD), lactic dehydrogenase (LD), diphosphopyridine nucleotide diaphorase (DPND), triphosphopyridine nucleotide diaphorase (TPND) and glucose-6-phosphate dehydrogenase (G-6-PD) in the pancreas from the American variety of obese-hyperglycemic mice (AO-mice) and their lean litter mates (AN-mice).
A high LD activity was found in the exocrine parenchyma, while the reaction in the islet tissue and the duct epithelium was only weak. A considerable reaction for DPND was noted throughout the pancreas. SD activity was slightly more pronounced in the acinar tissue and duct epithelium as compared to the islet tissue, where only a moderate activity appeared. Strong reactions for TPND and G-6-PD were found in the islet cells and duct epithelium, while the activity in the exocrine parenchyma was less pronounced. The hyperactive islet B cells in the AO-mice showed no obvious differences in enzyme activity and distribution compared to that of the AN-mice. The enzyme pattern of the A cells could not be clearly distinguished from that in the B cells.
The results suggest the existence at least in the B cells of the mice islet tissue of an active hexosemonophosphate shunt. The probable significance of the hexosemonophosphate shunt for insulin synthesis is briefly discussed.
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Abbreviations
- DPN:
-
Diphosphopyridine nucleotide
- DPND:
-
Diphosphopyridine nucleotide diaphorase
- DPNH:
-
Diphosphopyridine nucleotide, reduced form
- EM:
-
Embden-Meyerhof
- G-6-PD:
-
Glucose-6-phosphate dehydrogenase
- HMP:
-
Hexose monophosphate
- LD:
-
Lactic dehydrogenase
- MTT:
-
3,5-diphenyl-2-(4,5-dimethyl-thiazol-2-yl) tetrazolium bromide
- Nitro-BT:
-
2,2′-di-p-nitrophenyl-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-biphenylene)-ditetrazolium chloride
- PVP:
-
Polyvinyl pyrrolidone (M. W. 11 000)
- SD:
-
Succinic dehydrogenase
- TPN:
-
Triphosphopyridine nucleotide
- TPND:
-
Triphosphopyridine nucleotide diaphorase
- TPNH:
-
Triphosphopyridine nucleotide, reduced form
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Hellman, B., Hellerström, C. Oxidative enzymes in the pancreatic islets of normal and obese-hyperglycemic mice. Z. Zellforsch. 56, 97–106 (1962). https://doi.org/10.1007/BF00326849
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DOI: https://doi.org/10.1007/BF00326849