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Submicroscopic structure of the membranous labyrinth

III. The supporting structure of Corti's organ (basilar membrane, limbus spiralis and spiral ligament)

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Summary

Investigations were performed by light and electron microscope on the basilar membrane, limbus spiralis and spiral ligament.

These different parts continue one into the other and make up a single morphological and functional structure which may be called the “supporting structure of Corti's organ” (s.s.C.o.).

It is formed by a tissue the components of which are the cells and an intercellular substance in which are arranged the capillary vessels.

The cells can be classed in two groups, the first consisting of the cells proper (basilar membrane, limbus spiralis and spiral ligament cells) which present structural changes parallel with the growth mechanism of the intercellular substance; the second of the cochlear duct covering cells (Corti's organ cells, inner and outer spiral sulcus cells, interdental cells, stria vascularis cells).

The intercellular substance is organised in laminae, fibrolaminae, bundles and microscopic fibers composed of filaments with an intervening ground substance.

The filaments have a diameter ranging from 85 to 105 Å. Topochemical tests with polarised light microscope, enzymatic tests, diffractographic and chemical analyses suggest that these filaments unquestionably consist of protein material which have nothing to do with collagen or elastic fibers. Perhaps it may be classed in the K.E.M.F. group.

The ground substance generally appear anhistous and transparent but in some parts of the basilar membrane it presents a cottony appearance.

The possible different hypotheses about the classification of the s.s.C.o. tissue are discussed.

The quantity and architecture of the cells and the intercellular substance vary appreciably in the basilar membrane, limbus spiralis and spiral ligament, which are examined in detail one by one.

The demonstration that the s.s.C.o. is formed of a tissue possessing an intercellular substance containing filamentous scleroproteins clearly corroborates the theory that is performs supporting activity in respect of Corti's organ. The term “supporting structure of Corti's organ” is based on this interpretation.

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References

  • Agazzi, C.: Angioarchitettonica del legamento spirale della cavia. Biol. lat. (Milano) 1, 59–74 (1948/49).

    Google Scholar 

  • Amprino, R., e A. Bairati: Studi sulle trasformazioni delle cartilagini dell'uomo nell'accrescimento e nella senescenza. Z. Zellforsch. 20, 143–205 (1933).

    Google Scholar 

  • Baccetti, B.: Primi reperti sulla struttura submicroscopica dello stroma di sostegno di alcuni organi degli insetti. Atti Accad. Sci. Torino 95, 343–350 (1960/61).

    Google Scholar 

  • Bairati, A.: Struttura submicroscopica del connettivo aciculare. Arch. Sci. biol. (Bologna) 39, 31–58 (1955).

    Google Scholar 

  • —: The submicroscopic structure of collagen. Sci. med. ital. 4, 560–613 (1956).

    Google Scholar 

  • Bairati jr. A., e S. Iurato: Rricerche sulla organizzazione microscopica e submicroscopica del legamento spirale e della membrana basilare dell'organo del Corti. 2. Indagini a luce polarizzata ed al microscopio elettronico. Boll. Soc. ital. Biol. sper. 34, 187–188 (1958).

    Google Scholar 

  • —, e S. Iurato: Natura e struttura submicroscopica della membrana basale dei canali semicircolari. Monit. zool. ital. Suppl. 67, 242–247 (1959).

    Google Scholar 

  • —: Sulla struttura submicroscopica delle zone dell'epitelio del labirinto addette alla produzione dell'endolinfa. Monit. zool. ital. Suppl. 68, 39–43 (1960).

    Google Scholar 

  • Békésy, G. v.: Description of some mechanical properties of the organ of Corti. J. acoust. Soc. Amer. 25, 770–785 (1953).

    Google Scholar 

  • Boettcher, A.: Über Entwicklung und Bau des Gehörlabyrinths. Dresden: Blochmann 1869.

    Google Scholar 

  • Borghesan, E.: Struttura e vascolarizzazione del legamento spirale. Ipotesi sulla produzione linfatica cocleare. Atti della Clinica O.R.L. dell'Università di Palermo, 1947/48, p. 171–195. Palermo: Scuola tipogr. “Boccone del Povero” 1949.

    Google Scholar 

  • —: Idoneità della benderella soleata all'assorbimento dell'endolinfa cocleare. Estr. dagli Atti della Clinica O.R.L. dell'Università di Palermo, 1948/49, p. 1–25. Palermo: Scuola tipogr. “Boccone del Povero” (1950 a.)

    Google Scholar 

  • —: Zona vascolo-epiteliale della benderella di coniglio considerata probabile sorgente di endolinfa. Estr. dagli Atti della Clinica O.R.L. dell'Università di Palermo, 1948/49, p. 1–25. Palermo: Scuola tipogr. “Boccone del Povero” (1950 b).

    Google Scholar 

  • —: Modality of the cochlear humoral circulation. Laryngoscope (St.Louis) 67, 1266–1285(1957).

    Google Scholar 

  • Breschet, G.: Recherches anatomiques et physiologiques sur l'organe de l'ouïe et l'audition. Mem. Acad. roy. Med. (Paris) 5, 229–523 (1836).

    Google Scholar 

  • Cimino, A.: La benderella soleata nello sviluppo embriologico. Minerva otorinolaring. (Torino) 5, 331–339 (1955).

    Google Scholar 

  • Claudius, M.: Bemerkungen über den Bau der häutigen Spiralleiste der Schnecke. Z. wiss. Zool. 7, 154–161 (1856).

    Google Scholar 

  • Corey, R. B., and L. Pauling: The configuration of polypeptide chains in proteins. Istituto Lombardo (Rend. Sci.) 89, 10–37 (1955).

    Google Scholar 

  • Corti, A.: Recherches sur l'organe de l'ouïe des mammifères. Z. wiss. Zool. 3, 109–169 (1851).

    Google Scholar 

  • Dische, Z.: Methods of biochemical analysis, vol. 2, p. 313–358. New York: Interscience 1955.

    Google Scholar 

  • Ebner, V.: In Handbuch der Gewebelehre des Menschen, herausgeg. von A. Kolliker, Bd. III. Leipzig: Wilhelm Engelmann 1903.

    Google Scholar 

  • Ekholm, R., and F. S. Sjöstrand: The ultrastructural organisation of the mouse thyroid gland. J. ultrastruct. Res. 1, 178–199 (1957).

    Google Scholar 

  • Engström, H.: The structure of the basilar membrane. Acta oto-rhino-laryng. belg. 9, 531–542 (1955).

    Google Scholar 

  • —, F. S. Sjöstrand u. H. Spoendlin: Feinstruktur der Stria vascularis beim Meerschweinchen. Pract. oto-rhino-laryng. (Basel) 17, 69–79 (1955).

    Google Scholar 

  • —, and J. Wersäll: The ultrastructural organisation of the organ of Corti and of vestibular sensory epithelia. Exp. Cell Res. Suppl. 5, 460–492 (1958).

    Google Scholar 

  • Gottstein, J.: Quoted by Cimino 1955.

  • Guild, S. R.: The width of the basilar membrane. Science 65, 67–69 (1927).

    Google Scholar 

  • Held, H. v.: In Handbuch der normalen und pathologischen Physiologie, herausgeg. von A. Bethe, Bd. 11, S. 467–541. Berlin: Springer 1926.

    Google Scholar 

  • Hensen, V.: Zur Morphologie der Schnecke des Menschen und der Säugetiere. Z. wiss. Zool. 13, 481–512 (1863).

    Google Scholar 

  • Huschke, E.: Traité de splanchnologie et des organes des sens. Paris: Baillière 1845.

    Google Scholar 

  • Kölliker, A.: Eléments d'histologie humaine. Paris: Masson & Cie. 1856.

    Google Scholar 

  • Kolmer, W.: Gehörorgan. In Handbuch der Mikroskopischen Anatomie des Menschen, herausgeg. von W. v. Möllendorff, S. 362–366. Berlin: Springer 1927.

    Google Scholar 

  • Iurato, S.: Ricerche sulla organizzazione microscopica e submieroscopica del legamento spirale e della membrana basilare dell'organo del Corti. I. Dati generali di struttura microscopica. Boll. Soc. ital. Biol. sper. 34, 186–187 (1958a).

    Google Scholar 

  • —: Precisazioni sulla struttura microscopica e sulle variazioni diametriche della membrana basilare dell'organo del Corti. Anat. Anz. 105, 275–281 (1958 b).

    Google Scholar 

  • —: Alcune precisazioni sulla morfologia della membrana tectoria e della membrana basilare dell'organo del Corti. Monit. zool. ital. Suppl. 66, 388–390 (1958 c).

    Google Scholar 

  • - Ultrastruttura e composizione chimica del lembo spirale, della membrana basilare, del legamento spirale e della membrana basale dei canali semioircolari. Atti del 48° Congr. Soc. ital. di Laring. Otol. e Rinologia, Firenze 1960 a.

  • —: Submicroscopic structure of the membranous labyrinth. 1. The tectorial membrane. Z. Zellforsch. 51, 105–128 (1960 b).

    Google Scholar 

  • —: Submicroscopic structure of the membranous labyrinth. 2. The epithelium of Corti's organ. Z. Zellforsch. 53, 259–298 (1961).

    Google Scholar 

  • -, and S. de Petris: Submicroscopic structure and nature of the limbus spiralis, the basilar membrane and the spiral ligament. Proc. Europ. Reg. Conf. on Electron Microscopy, vol. 2, p. 814–817. Delft 1960.

  • Lavdowsky, M.: Untersuchungen über den akustischen Endapparat der Säugethiere. Arch. mikr. Anat. 13, 497–557 (1877).

    Google Scholar 

  • Lawrence, M.: Structures of the spiral prominence and external sulcus and their relation to the organ of Corti. Trans. Amer. laryng. rhin. otol. Soc. 527–540 (1956).

  • Mangabeira-Albernaz, P. L.: Histochemistry of the connective tissue of the cochlea. Laryngoscope (St. Louis) 71, 1–18 (1961).

    Google Scholar 

  • Mussini, E.: Nuovo rapido metodo di dosaggio dei gruppi sulfidrilici in materiali biologici. Boll. Soc. ital. Biol. sper. 34, 1419–1421 (1958).

    Google Scholar 

  • Palay, S. L., and G. E. Palade: The fine structure of neurons. J. biophys. biochem. Cytol. 1, 69–88 (1955).

    Google Scholar 

  • Pasini, C.: Sulla struttura morfologica della membrana basilare della cavia. Arch. ital. Otol. 55, 147–160 (1943).

    Google Scholar 

  • Pedersen, K. J.: Studies on the nature of Planarian connective tissue. Z. Zellforsch. 53, 569–608 (1961).

    Google Scholar 

  • Pernis, B., e S. Iurato: Ricerche sulla organizzazione microscopica e submicroscopica del legamento spirale e della membrana basilare dell'organo del Corti. III. Indagini diffrattografiche e chimiche. Boll. Soc. ital. Biol. sper. 34, 189–191 (1958).

    Google Scholar 

  • —, and Ch. Wunderly: Quantitative determination of amino acids on filter paper. Biochim. biophys. Acta 11, 209–214 (1953).

    Google Scholar 

  • Plotz, E., and H. B. Perlman: A histochemical study of the cochlea. Laryngoscope (St. Louis) 65, 291–312 (1955).

    Google Scholar 

  • Retzius, G.: Das Gehörorgan der Wirbeltiere. 2. Das Gehörorgan der Reptilien, der Vögel und der Säugetiere, S. 1–368. Stockholm 1884.

  • Rhodin, J.: Electron microscopy of the glomerular capillary wall. Exp. Cell Res. 8, 572–574 (1955).

    Google Scholar 

  • Ruthmann, A.: Basophilic lamellar systems in the Crayfish spermatocyte. J. biophys. biochem. Cytol. 4, 267–274 (1958).

    Google Scholar 

  • Scuderi, R., e M. del Bo: La vascolarizzazione del labirinto umano. Arch. ital. Otol. Suppl. 11, 1–90 (1952).

    Google Scholar 

  • Smith, C. A.: Capillary areas of the membranous labyrinth. Ann. Otol. (St. Louis) 63, 435–447 (1954).

    Google Scholar 

  • —: Electron microscopic studies of the organ of Corti. Anat. Rec. 121, 451 (1955).

    Google Scholar 

  • —: Structure of the stria vascularis and the spiral prominence. Ann. Otol. (St. Louis) 66, 521–536 (1957).

    Google Scholar 

  • —, and E. W. Dempsey: Electron microscopy of the organ of Corti. Amer. J. Anat. 100, 337–367 (1957).

    Google Scholar 

  • Spoendlin, H.: Elektronenmikroskopische Untersuchungen am Cortischen Organ des Meerschweinchens. Pract. oto-rhino-laryng. (Basel) 19, 192–234 (1957).

    Google Scholar 

  • Stricht, O. van der: Quoted by Cimino 1955.

  • Todd, R. B., and W. Bowman: The physiological anatomy and physiology of man, vol. II. London: Parker and Son 1859.

    Google Scholar 

  • Trier, J. S.: The fine structure of the parathyroid gland. J. biophys. biochem. Cytol. 4, 13–22 (1958).

    Google Scholar 

  • Troll, W., and R. K. Cannan: A modified photometric ninhydrin method for the analysis of amino and imino acids. J. biol. Chem. 200, 803–811 (1953).

    Google Scholar 

  • Wada, T.: Quoted by Wever 1938.

  • Walker, B. E.: Electron microscopic observations on transitional epithelium of the mouse urinary bladder. J. ultrastruct. Res. 3, 345–361 (1960).

    Google Scholar 

  • Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).

    Google Scholar 

  • Wever, E. G.: The width of the basilar membrane in man. Ann. Otol. (St. Louis) 47, 37–47 (1938).

    Google Scholar 

  • Wrightson, T., and A. Keith: Quoted by Wever 1938.

  • Yamada, E.: The fine structure of the renal glomerulus of the mouse. J. biophys. biochem. Cytol. 1, 551–566 (1955).

    Google Scholar 

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  1. Institute of Normal Human Anatomy of the University of Milan, Italy

    Salvatore Iurato

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  1. Salvatore Iurato
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Iurato, S. Submicroscopic structure of the membranous labyrinth. Z. Zellforsch. 56, 40–96 (1962). https://doi.org/10.1007/BF00326848

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