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A simple method for preparing meiotic chromosomes from mammalian testis

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Abstract

Freshly removed testis tubules are treated with hypotonic citrate solution and fixed in 3∶1 acetic alcohol without undue rupture. The material is transferred to 60% acetic acid and the resulting suspension is air-dried onto warm slides using a micropipette. Finally the slides are stained with lacto-acetic orcein. — Provided suitable storage is made, slides need not be prepared in the same week as fixation and this may facilitate the collection of material during field study.

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References

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Authors and Affiliations

  1. Radiobiological Research Unit, Medical Research Council, Harwell, Didcot, Berkshire

    R. Meredith

  2. Department of Biology as Applied to Medicine, The Middlesex Hospital Medical School, W. 1., London, UK

    R. Meredith

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  1. R. Meredith
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Meredith, R. A simple method for preparing meiotic chromosomes from mammalian testis. Chromosoma 26, 254–258 (1969). https://doi.org/10.1007/BF00326520

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  • DOI: https://doi.org/10.1007/BF00326520

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