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The nuclear gene and cDNAs encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa: cloning, characterization and phylogenetic analysis

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Abstract

We have cloned and sequenced the single-copy nuclear gene (GapC) encoding the complete 335-amino acid cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) from the red alga Gracilaria verrucosa. The proline residue which contributes to the specificity of NAD+ binding in other GAPC-like proteins is present. Putative regulatory regions, including GC-rich regions, a GATA element, and 11-base T- and T/G-clusters, but excluding TATA- and CCAAT-boxes, were identified upstream. Two types of GapC cDNAs differing in polyadenylation site were characterized. An 80-bp phase-two spliceosomal intron was identified in a novel position interrupting the highly conserved cofactor-coding region I. The G. verrucosa GAPC was easily aligned with other known GAPC-type sequences. Inferred phylogenetic trees place red algae among the eukaryote crown taxa, although with modest bootstrap support and without stable resolution among related GAPC lineages.

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Authors and Affiliations

  1. Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, B3H 3Z1, Halifax, Nova Scotia, Canada

    Yi-Hong Zhou & Mark A. Ragan

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  1. Yi-Hong Zhou
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  2. Mark A. Ragan
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Communicated by R.W. Lee

GSDB accession L38661 Issued as NRCC no. 38071

Present address Human Genetics Center, University of Texas, P.O. Box 20334, Houston, TX 77225, USA

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Zhou, YH., Ragan, M.A. The nuclear gene and cDNAs encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa: cloning, characterization and phylogenetic analysis. Curr Genet 28, 324–332 (1995). https://doi.org/10.1007/BF00326430

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  • DOI: https://doi.org/10.1007/BF00326430

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