, Volume 47, Issue 4, pp 353–359 | Cite as

Localization of the 5S RNA genes in Zea mays by RNA-DNA hybridization in situ

  • D. E. Wimber
  • Patricia A. Duffey
  • D. M. Steffensen
  • W. Prensky


5S RNA was extracted from Zea mays tissue and iodinated in vitro with 125I to a high specific activity. Acrylamide gel electrophoresis of the 125I-5S RNA, 11/2 weeks after iodination demonstrated that most of the 5S RNA molecules were degraded to half-size or smaller. In situ hybridization with this iodinated RNA to pachytene microsporocyte chromosomes showed that the 5S RNA cistrons are located near the end of the long arm of chromosome 2. No obvious association of the 5S locus with the nucleolus was seen during pachytene or later stages.


Developmental Biology Acrylamide High Specific Activity Obvious Association 
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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • D. E. Wimber
    • 1
  • Patricia A. Duffey
    • 1
  • D. M. Steffensen
    • 2
  • W. Prensky
    • 3
  1. 1.Department of BiologyUniversity of OregonEugeneUSA
  2. 2.Provisional Department of Genetics and DevelopmentUniversity of IllinoisUrbanaUSA
  3. 3.Sloan Kettering InstituteNew YorkUSA

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