Summary
A new method is introduced for packing micro-columns with an ID (inner diameter) of 320 μm. The advantage of this method is the extraordinary economy of the packing material employed and the high reliability in production. The theoretical plate height ranges from 7 to 8 μm for 3 μm particles.
References
Menet H, Gareil P, Caude M, Rosset R (1984) Chromatographia 18:73
Shelly DC, Edkins TJ (1987) J Chromatogr 411:185
Ishii D, Asai A, Hibi K, Jonokuchi T, Nagaya M (1977) J Chromatogr 144:157
Vadukul NK, Loscombe CR (1983) HRC & CC 6:488
Vadukul NK, Loscombe CR (1982) HRC & CC 5:360
Karlson K-E, Novotny M (1988) Anal Chem 60:1662
Kuwata K, Uebori M, Yamazaki Y (1981) J Chromatogr 211:378
Welling P, Poppe H, Kraak JC (1985) J Chromatogr 321:450
Yang FJ (1989) Microbore column chromatography. Chromatographic science series 45:56
Unger KK, Eray B (1992) GIT spezial. Chromatographie 1:5
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bächmann, K., Haag, I. & Prokop, T. Modified method for filling micro-HPLC-columns. Fresenius J Anal Chem 345, 545–546 (1993). https://doi.org/10.1007/BF00326349
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00326349