Abstract
The location of the 5S ribosomal RNA cistrons in band 2-23B1,2 of the polytene (salivary gland) chromosomes of Drosophila hyaei was indicated by in situ hybridization of tritiated low molecular weight RNA fractionated from total in vivo synthesized larval RNA or from in vitro synthesized salivary gland RNA and competition of the hybridization of this RNA by 5S RNA obtained from calf lens ribosomes. —At the submicroscopic level, band 2-23B1,2 in salivary gland chromosomes shows a compact organization. The adjacent region, 23B2, is slightly puffed and displays typical RNP particles, some of which may be observed close to band 2-23B1,2.
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Alonso, C.: Improved conditions for in situ RNA/DNA hybridization. FEBS Letters 31, 85–88 (1973)
Alonso, C., Grootegoed, J. A.: RNA synthesis in polytene salivary glands of Drosophila hydei maintained under in vitro conditions. Cell Different. 2, 107–118 (1973)
Alonso, C., Helmsing, P. J., Berendes, H. D.: A comparative study of in situ hybridization procedures using cRNA applied to Drosophila hydei salivary gland chromosomes. Exp. Cell Res. 85, 383–390 (1974)
Berendes, H. D.: The salivary gland chromosomes of Drosophila hydei Sturtevant. Chromosoma (Berl.) 14, 195–206 (1963)
Berendes, H. D.: The control of puffing in Drosophila hydei. In: Results and problems in cell diferentiation, vol. 4 (W. Beermann, ed.), p. 181–207. Berlin-Heidelberg-New York: Springer 1972
Berendes, H. D., Alonso, C., Helmsing, P. J., Leenders, H. J., Derksen, J.: Structure and function in the genome of Drosophila hydei. Cold Spr. Harb. Symp. quant. Biol. 38, 645–654 (1973)
Berendes, H. D., Meyer, G.-F.: A specific chromosome element, the telomere of Drosophila polytene chromosomes. Chromosoma (Berl.) 25, 184–197 (1968)
Birnstiel, M. L., Weinberg, E. S., Pardue, M. L.: Evoution of 9S mRNA genes. In: Molecular cytogenetics (B. A. Hamkalo and J. Pappaconstantinou, eds.), p. 75–93. New York: Plenum Publ. Corp. 1973
Bishop, D. H. L., Claybrook, J. R., Spiegelman, S.: Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J. molec. Biol. 26, 373–387 (1967)
Brown, D. D., Wensink, P. C., Jordan, E.: Purification and some characteristics of 5S DNA from Xenopus laevis. Proc. nat. Acad. Sci. (Wash.) 68, 3175–3179 (1971)
Derksen, J.: The submicroscopic structure of synthetically active units in a puff of Drosophila hydei giant chromosomes. Chromosoma (Berl.) 50, 45–52 (1975)
Grigliatti, T. A., White, B. N., Tener, G. M., Kaufman, T. C., Holden, J. J., Suzuki, D. T.: Studies on the transfer RNA genes in Drosophila. Cold Spr. Harb. Symp. quant. Biol. 38, 461–474 (1973)
Grigliatti, T. A., White, B. N., Terner, G. N., Kaufman, T. C., Suzuki, D. T.: The localization of transfer RNA lyss genes in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 71, 3527–3531 (1974)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)
Pardue, M. L., Weinberg, E., Birnstiel, M. L., Kedes, L. H.: In: Modern aspects of cytogenetics: Constitutive heterochromatin. In: Man. Symp. Medica Hoechst (R. A. Pfeiffer, ed.), p. 75. Stuttgart: Schattauer 1973
Poels, C. L. M.: Mucopolysaccharide secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity. Cell Different. 1, 63–78 (1972)
Prensky, W., Steffensen, D. M., Hughes, W. L.: The use of iodinated RNA for gene localization. Proc. nat. Acad. Sci. (Wash.) 70, 1860–1864 (1973)
Ritossa, F. M., Atwood, K. C., Spiegelman, S.: A molecular explanation of the bobbed mutants of Drosophila as partial deficiencies of “ribosomal” DNA. Genetics 54, 819–834 (1966)
Sorsa, M.: Ultrastructure of the chromocenter heterochromatin in Drosophila melanogaster. Ann. Acad. Sci. Fenn. Ser. A IV, Biol. 146, 1–20 (1969a)
Sorsa, M.: Ultrastructure of puffs in the proximal part of chromosome 3R in Drosophila melanogaster. Ann. Acad. Sci. Fenn. Ser. A IV, Biol. 150, 1–21 (1969b)
Sorsa, V.: Ultrastructure of the 5S RNA locus in salivary gland chromosomes of Drosophila melanogaster. Hereditas (Lund) 74, 297–301 (1973)
Steffensen, D. M., Wimber, D. E.: Localization of tRNA genes in the salivary chromosomes of Drosophila by RNA∶DNA hybridization. Genetics 69, 163–187 (1971)
Steffensen, D. M., Wimber, D. E.: Hybridization of nuclei acids to chromosomes. In: Results and problems in cell differentiation, vol. 3 (H. Ursprung, ed.), p. 47–63. Berlin-Heidelberg-New York: Springer 1972
Tartof, K. D., Perry, R. P.: The 5S genes of Drosophila melanogaster. J. molec. Biol. 51, 171–183 (1970)
Weinberg, R.: Regulation of ribosomal RNA and 5S RNA synthesis in Drosophila melanogaster. I. Bobbed mutants. Genetics 72, 267–276 (1972)
Wimber, D. E., Steffensen, D. M.: Localization of 5S RNA genes on Drosophila chromosomes by RNA/DNA hybridization. Science 170, 639–641 (1970)
Wimber, D. E., Steffensen, D. M.: Localization of gene function. In: Ann. Rev. Genetics, vol. 7, p. 205–223 (H. L. Roman, L. M. Sandler and A. Campbell, eds.). Palo Alto: Annual. Reviews Inc. 1973
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Alonso, C., Berendes, H.D. The location of 5S (ribosomal) RNA genes in Drosophila hydei . Chromosoma 51, 347–356 (1975). https://doi.org/10.1007/BF00326321
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DOI: https://doi.org/10.1007/BF00326321