Skip to main content
Log in

The location of 5S (ribosomal) RNA genes in Drosophila hydei

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

The location of the 5S ribosomal RNA cistrons in band 2-23B1,2 of the polytene (salivary gland) chromosomes of Drosophila hyaei was indicated by in situ hybridization of tritiated low molecular weight RNA fractionated from total in vivo synthesized larval RNA or from in vitro synthesized salivary gland RNA and competition of the hybridization of this RNA by 5S RNA obtained from calf lens ribosomes. —At the submicroscopic level, band 2-23B1,2 in salivary gland chromosomes shows a compact organization. The adjacent region, 23B2, is slightly puffed and displays typical RNP particles, some of which may be observed close to band 2-23B1,2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alonso, C.: Improved conditions for in situ RNA/DNA hybridization. FEBS Letters 31, 85–88 (1973)

    Google Scholar 

  • Alonso, C., Grootegoed, J. A.: RNA synthesis in polytene salivary glands of Drosophila hydei maintained under in vitro conditions. Cell Different. 2, 107–118 (1973)

    Google Scholar 

  • Alonso, C., Helmsing, P. J., Berendes, H. D.: A comparative study of in situ hybridization procedures using cRNA applied to Drosophila hydei salivary gland chromosomes. Exp. Cell Res. 85, 383–390 (1974)

    Google Scholar 

  • Berendes, H. D.: The salivary gland chromosomes of Drosophila hydei Sturtevant. Chromosoma (Berl.) 14, 195–206 (1963)

    Google Scholar 

  • Berendes, H. D.: The control of puffing in Drosophila hydei. In: Results and problems in cell diferentiation, vol. 4 (W. Beermann, ed.), p. 181–207. Berlin-Heidelberg-New York: Springer 1972

    Google Scholar 

  • Berendes, H. D., Alonso, C., Helmsing, P. J., Leenders, H. J., Derksen, J.: Structure and function in the genome of Drosophila hydei. Cold Spr. Harb. Symp. quant. Biol. 38, 645–654 (1973)

    Google Scholar 

  • Berendes, H. D., Meyer, G.-F.: A specific chromosome element, the telomere of Drosophila polytene chromosomes. Chromosoma (Berl.) 25, 184–197 (1968)

    Google Scholar 

  • Birnstiel, M. L., Weinberg, E. S., Pardue, M. L.: Evoution of 9S mRNA genes. In: Molecular cytogenetics (B. A. Hamkalo and J. Pappaconstantinou, eds.), p. 75–93. New York: Plenum Publ. Corp. 1973

    Google Scholar 

  • Bishop, D. H. L., Claybrook, J. R., Spiegelman, S.: Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J. molec. Biol. 26, 373–387 (1967)

    Google Scholar 

  • Brown, D. D., Wensink, P. C., Jordan, E.: Purification and some characteristics of 5S DNA from Xenopus laevis. Proc. nat. Acad. Sci. (Wash.) 68, 3175–3179 (1971)

    Google Scholar 

  • Derksen, J.: The submicroscopic structure of synthetically active units in a puff of Drosophila hydei giant chromosomes. Chromosoma (Berl.) 50, 45–52 (1975)

    Google Scholar 

  • Grigliatti, T. A., White, B. N., Tener, G. M., Kaufman, T. C., Holden, J. J., Suzuki, D. T.: Studies on the transfer RNA genes in Drosophila. Cold Spr. Harb. Symp. quant. Biol. 38, 461–474 (1973)

    Google Scholar 

  • Grigliatti, T. A., White, B. N., Terner, G. N., Kaufman, T. C., Suzuki, D. T.: The localization of transfer RNA lyss genes in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 71, 3527–3531 (1974)

    Google Scholar 

  • Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Pardue, M. L., Weinberg, E., Birnstiel, M. L., Kedes, L. H.: In: Modern aspects of cytogenetics: Constitutive heterochromatin. In: Man. Symp. Medica Hoechst (R. A. Pfeiffer, ed.), p. 75. Stuttgart: Schattauer 1973

    Google Scholar 

  • Poels, C. L. M.: Mucopolysaccharide secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity. Cell Different. 1, 63–78 (1972)

    Google Scholar 

  • Prensky, W., Steffensen, D. M., Hughes, W. L.: The use of iodinated RNA for gene localization. Proc. nat. Acad. Sci. (Wash.) 70, 1860–1864 (1973)

    Google Scholar 

  • Ritossa, F. M., Atwood, K. C., Spiegelman, S.: A molecular explanation of the bobbed mutants of Drosophila as partial deficiencies of “ribosomal” DNA. Genetics 54, 819–834 (1966)

    Google Scholar 

  • Sorsa, M.: Ultrastructure of the chromocenter heterochromatin in Drosophila melanogaster. Ann. Acad. Sci. Fenn. Ser. A IV, Biol. 146, 1–20 (1969a)

    Google Scholar 

  • Sorsa, M.: Ultrastructure of puffs in the proximal part of chromosome 3R in Drosophila melanogaster. Ann. Acad. Sci. Fenn. Ser. A IV, Biol. 150, 1–21 (1969b)

    Google Scholar 

  • Sorsa, V.: Ultrastructure of the 5S RNA locus in salivary gland chromosomes of Drosophila melanogaster. Hereditas (Lund) 74, 297–301 (1973)

    Google Scholar 

  • Steffensen, D. M., Wimber, D. E.: Localization of tRNA genes in the salivary chromosomes of Drosophila by RNA∶DNA hybridization. Genetics 69, 163–187 (1971)

    Google Scholar 

  • Steffensen, D. M., Wimber, D. E.: Hybridization of nuclei acids to chromosomes. In: Results and problems in cell differentiation, vol. 3 (H. Ursprung, ed.), p. 47–63. Berlin-Heidelberg-New York: Springer 1972

    Google Scholar 

  • Tartof, K. D., Perry, R. P.: The 5S genes of Drosophila melanogaster. J. molec. Biol. 51, 171–183 (1970)

    Google Scholar 

  • Weinberg, R.: Regulation of ribosomal RNA and 5S RNA synthesis in Drosophila melanogaster. I. Bobbed mutants. Genetics 72, 267–276 (1972)

    Google Scholar 

  • Wimber, D. E., Steffensen, D. M.: Localization of 5S RNA genes on Drosophila chromosomes by RNA/DNA hybridization. Science 170, 639–641 (1970)

    Google Scholar 

  • Wimber, D. E., Steffensen, D. M.: Localization of gene function. In: Ann. Rev. Genetics, vol. 7, p. 205–223 (H. L. Roman, L. M. Sandler and A. Campbell, eds.). Palo Alto: Annual. Reviews Inc. 1973

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Alonso, C., Berendes, H.D. The location of 5S (ribosomal) RNA genes in Drosophila hydei . Chromosoma 51, 347–356 (1975). https://doi.org/10.1007/BF00326321

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00326321

Keywords

Navigation