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The chromosomal distribution of satellite DNA in the germ-line and somatic tissues of the gall midge, Heteropeza pygmaea

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Abstract

Somatic DNA from Heteropeza pygmaea separated in CsCl gradients into a main band DNA (ϱ=1.685 g/cm3) and a satellite band (ϱ=1.716 g/cm3) comprising 15% of the total DNA. The satellite melted sharply at 93.0°C in SSC, 10.4°C higher than the main band DNA. Satellite DNA reassociated rapidly, banding in CsCl heavier than native satellite but lighter than denatured satellite. The complementary strands of the satellite formed a single band in alkaline gradients and hence are apparently similar in G+T composition. — Filter hybridization experiments with Xenopus ribosomal RNA showed that the satellite band does not contain ribosomal cistrons. — Complementary RNA (cRNA) transcribed in vitro from isolated satellite bound extensively to satellite DNA but not to main band DNA. — The strain of Heteropeza used here contained about 58 chromosomes in germ-line cells and reproduced only paedogenetically. During early cleavage, the presumptive somatic nuclei eliminate most of their chromosomes (E-chromosomes) and retain only ten (S-chromosomes). In situ hybridizations with satellite-cRNA showed satellite DNA (prepared from predominately somatic tissues) to be localized in the centromeric heterochromatin of S- and E-chromosomes. Silver grain comparisons suggested that the amount of satellite is equivalent in both types of chromosomes. — Lastly we found that both diploid and polyploid cells contain similar amounts of satellite DNA. We interpreted this to mean that during polyploidization, as has been demonstrated during polytenization, satellite DNA does not replicate or replicates only slightly while other DNA fractions increase.

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References

  • Arrighi, F. E., Hsu, T. C.: Localization of heterochromatin in human chromosomes. Cytogenetics 10, 81–86 (1971)

    Google Scholar 

  • Bantock, C.: Chromosome elimination in Cecidomyidae. Nature (Lond.) 190, 466–467 (1961)

    Google Scholar 

  • Brown, D. D., Dawid, I. B.: Specific gene amplification in oocytes. Science 160, 272–280 (1968)

    Google Scholar 

  • Burgess, R. R.: A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J. biol. Chem. 244, 6160–6167 (1969)

    Google Scholar 

  • Camenzind, R.: Die Zytologie der bisexuellen und parthenogenetischen Fortpflanzung von Heteropeza pygmaea Winnertz, einer Gallmücke mit pädogenetischer Vermehrung. Chromosoma (Berl.) 18, 123–152 (1966)

    Google Scholar 

  • Dickson, E., Boyd, J. B., Laird, C. D.: Sequence diversity of polytene chromosome DNA from Drosophila hydei. J. molec. Biol. 61, 615–627 (1971)

    Google Scholar 

  • Eckhardt, R. A.: Chromosomal localization of repetitive DNA. In: Evolution of genetic systems (H. H. Smith, ed.). Brookhaven Symp. Biol. 23, 271–292 (1972)

  • Eckhardt, R. A., Gall, J. G.: Satellite DNA associated with heterochromatin in Rhynchosciara. Chromosoma (Berl.) 32, 407–427 (1971)

    Google Scholar 

  • Evans, D., Birnstiel, M. L.: Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis. Biochim. biophys. Acta (Amst.) 166, 274–276 (1968)

    Google Scholar 

  • Flamm, W. G., McCallum, M., Walker, P. M. B.: The isolation of complementary strands from a mouse DNA fraction. Proc. nat. Acad. Sci. (Wash.) 57, 1729–1734 (1967)

    Google Scholar 

  • Gall, J. G.: Differential synthesis of the genes for ribosomal RNA during amphibian oogenesis. Proc. nat. Acad. Sci. (Wash.) 60, 553–560 (1968)

    Google Scholar 

  • Gall, J. G.: The genes for ribosomal RNA during oogenesis. Genetics 61, (Suppl.), 121–132 (1969)

    Google Scholar 

  • Gall, J. G., Cohen, E. H., Polan, M. L.: Repetitive DNA sequences in Drosophila. Chromosoma (Berl.) 33, 319–344 (1971)

    Google Scholar 

  • Gall, J. G., Pardue, M. L.: Nucleic acid hybridization in cytological preparations. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), vol. XII D, p. 470–480. New York: Academic Press Inc. 1971

    Google Scholar 

  • Geyer-Duszyńska, I.: Genetic factors in oögenesis and spermatogenesis in Cecidomyiidae. In: Chromosomes today 1, 174–178 (1966)

    Google Scholar 

  • Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol. 12, 829–842 (1965)

    Google Scholar 

  • Hagan, H.: Embryology of the viviparous insects. New York: Ronald Press 1951

    Google Scholar 

  • Hauschteck, E.: Die Zytologie der Pädogenese und der Geschlechtsbestimmungeiner heterogonen Gallmücke. Chromosoma (Berl.) 13, 163–182 (1962)

    Google Scholar 

  • Hennig, W.: Highly repetitive DNA sequences in the genome of Drosophila hydei. II. Occurrence in polytene tissues. J. molec. Biol. 71, 419–431 (1972)

    Google Scholar 

  • Hennig, W., Meer, B.: Reduced polytene of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature (Lond.) New Biol. 233, 70–72 (1971)

    Google Scholar 

  • Kraczkiewicz, Z.: Premiers stades de l'oogenèse de Rhabdophaga saliciperda (Cecidomyiidae, Diptera). Chromosoma (Berl.) 18, 208–229 (1966)

    Google Scholar 

  • Kunz, W.: Genetische Aktivität der Keimbahnchromosomen während des Eiwachstums von Gallmücken (Cecidomyiidae). Verh. Dtsch. Zool. Ges. 64, 42–46 (1970)

    Google Scholar 

  • Kunz, W., Trepte, H. H., Bier, K.: On the function of the germ line chromosomes in the oogenesis of Wachtliella persicariae (Cecidomyiidae). Chromosoma (Berl.) 30, 180–192 (1970)

    Google Scholar 

  • Marmur, J., Doty, P.: Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. molec. Biol. 5, 109–118 (1962)

    Google Scholar 

  • Matuszewski, B.: Oogenesis in Mikiola fagi Hart. (Cecidomyiidae; Diptera). Chromosoma(Berl.) 12, 741–811 (1962)

    Google Scholar 

  • Metcalfe, M. E.: The germ-cell cycle in Phytophaga destructor Say. Quart. J. micr. Sci. 77, 585–603 (1935)

    Google Scholar 

  • Nicklas, R. B.: The germ-cell cycle in Phytophaga destructor Say. Quart. J. micr.

  • Nicklas, R. B.: An experimental and descriptive study of chromosome elimination in Miastor spec. (Cecidomyidae; Diptera). Chromosoma (Berl.) 10, 301–336 (1959)

    Google Scholar 

  • Nicklas, R. B.: The chromosome cycle of a primitive cecidomyiid — Mycophila speyeri. Chromosoma (Berl.) 11, 402–418 (1960)

    Google Scholar 

  • Panelius, S.: Male germ line, spermatogenesis and karyotypes of Heteropeza Winnertz (Diptera: Cecidomyiidae). Chromosoma (Berl.) 32, 295–331 (1971)

    Google Scholar 

  • Pardue, M. L., Gerbi, S. A., Eckhardt, R. A., Gall, J. G.: Cytological localization of DNA complementary to ribosomal RNA in poyltene chromosomes of Diptera. Chromosoma (Berl.) 29, 268–290 (1970)

    Google Scholar 

  • Reitberger, A.: Die Cytologie des pädogenetischen Entwicklungszyklus der Gallmücke Oligarces paradoxus Mein. Chromosoma (Berl.) 1, 391–473 (1940)

    Google Scholar 

  • Ritossa, F. M.: Unstable redundancy of genes for ribosomal RNA. Proc. nat. Acad. Sci. (Wash). 60, 509–516 (1968)

    Google Scholar 

  • Ritossa, F.: Procedure for magnification of lethal deletions of genes for ribosomal RNA. Nature (Lond.) New Biol. 240, 109–111 (1972)

    Google Scholar 

  • Schildkraut, C. L., Marmur, J., Doty, P.: Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. molec. Biol. 4, 430–443 (1962)

    Google Scholar 

  • Sinclair, J. H., Brown, D. D.: Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics. Biochemistry 10, 2761–2769 (1971)

    Google Scholar 

  • Spear, B. B., Gall, J. G.: Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 70, 1359–1363 (1973)

    Google Scholar 

  • Ulrich, H.: Experimentelle Untersuchungen über den Generationswechsel der heterogonen Cecidomyide Oligarces paradoxus. Z. indukt. Abstamm.-u. Vererb.-Lehre 71, 1–60 (1936)

    Google Scholar 

  • Vinograd, J., Morris, J., Davidson, N., Dove, W.: The buoyant behavior of viral and bacterial DNA in alkaline CsCl. Proc. nat. Acad. Sci. (Wash.) 49, 12–17 (1963)

    Google Scholar 

  • Walker, P. M. B.: “Repetitive” DNA in higher organisms. In: Progress in biophysics and molecular biology (J. A. V. Butler and D. Noble, eds. vol. 23, p. 145–190. New York: Pergamon Press 1971

    Google Scholar 

  • White, M. J. D.: Cytological studies on gall midges (Cecidomyidae). Univ. Texas Publ. No. 5007, p. 1–80 (1950)

  • White, M. J. D.: Animal cytology and evolution, 2nd edit. Cambridge: Cambridge University Press 1954

    Google Scholar 

  • Yunis, J. J., Yasmineh, W. G.: Heterochromatin, satellite DNA, and cell function. Science 174, 1200–1209 (1971)

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Yale University, USA

    Werner Kunz & Ronald A. Eckhardt

  2. Department of Biology, Brooklyn College of The City University of New York, USA

    Werner Kunz & Ronald A. Eckhardt

  3. Institut für Allgemeine Biologie, Universität Düsseldorf, Ulenbergstr. 127, 4, Düsseldorf, Federal Republic of Germany

    Werner Kunz

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  1. Werner Kunz
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  2. Ronald A. Eckhardt
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Kunz, W., Eckhardt, R.A. The chromosomal distribution of satellite DNA in the germ-line and somatic tissues of the gall midge, Heteropeza pygmaea . Chromosoma 47, 1–19 (1974). https://doi.org/10.1007/BF00326268

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  • DOI: https://doi.org/10.1007/BF00326268

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