Skip to main content
Log in

A genetic analysis of an alpha-amylase super-secretor in yeast; implications for the regulatory pathway

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

Extracellular glucoamylase activity was increased by a gene, which is present in super-secretor, but absent in low-secretor, strains of the yeast Saccharomyces cerevisiae. Genetic data indicated that this super-secretor gene is linked to the STA3 structural gene for glucoamylase. This gene appears to act specifically since it increased the secretion of glucoamylase but not of other secreted enzymes like acid phosphatase and invertase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barnett JA, Payne RW, Yarrow D (1990) Yeast: characteristics and identification. 2nd edn. Cambridge University Press, England

    Google Scholar 

  • Calleja GB, Nasim A, Lusena CV, Levy-Rick S (1986) Biochem Internat 12:81–87

    Google Scholar 

  • Dohmen RJ, Strasser AWM, Zitomer RS, Hollenberg CP, (1989) Curr Genet 15:319–325

    Google Scholar 

  • Dranginis AM (1989) Mol Cell Biol 9:3992–3998

    Google Scholar 

  • Goldstein A, Lampen JO (1975) Methods Enzymol 42:504–511

    Google Scholar 

  • Hawthorne DC, Mortimer RK (1968) Genetics 60:735–745

    Google Scholar 

  • Jamieson J, Palade G (1967a) J Cell Biol 34:597–615

    Google Scholar 

  • Jamieson J, Palade G (1968b) J Cell Biol 34:597–615

    Google Scholar 

  • Novick P, Field CH, Scheckman R (1980) Cell 21:205–215

    Google Scholar 

  • Palade G (1975) Science 189:347–358

    Google Scholar 

  • Polaina J, Wiggs MY (1983) Curr Genet 7:109–111

    Google Scholar 

  • Pugh TA, Clancy MJ (1990) Mol Gen Genet 222:87–96

    Google Scholar 

  • Sakai A, Shimizu Y, Hishinuma F (1988) Genetics 119:499–506

    Google Scholar 

  • Salminen A, Novick PJ (1989) J Cell Biol 109:1023–1026

    Google Scholar 

  • Scheckman R, Novick PJ (1982) In: Strather J, Iones E, Broach J (eds) The molecular biology of yeast Saccharomyces: metabolism and gene expression. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York, pp 361–393

    Google Scholar 

  • Schurr A, Yagil E (1971) Regulation and characterization of acid and alkaline phosphatase in yeast. J Gen Microbiol 65:291–303

    Google Scholar 

  • Sherman F, Fink GR, Hicks JB (1981) Methods in yeast genetics: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York

    Google Scholar 

  • Smith RA, Ducan MJ, Moir TD (1985) Science, pp 1219–1224

  • Yamashita J, Takano Y, Fukui S (1985) J Bacteriol 164:769–773

    Google Scholar 

  • Wickerham LJ (1946) J Bacteriol 52:293–301

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. K. Zimmermann

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kotylak, Z., El-Gewely, M.R. A genetic analysis of an alpha-amylase super-secretor in yeast; implications for the regulatory pathway. Curr Genet 20, 181–184 (1991). https://doi.org/10.1007/BF00326229

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00326229

Key words

Navigation