Abstract
We have constructed a yeast mitochondrial genome containing only one group-I intron, r1, from the 21s rRNA gene and introduced this genome into a strain bearring a disruption of the suv3 gene. The presence of the r1 intron alone causes a block in respiration, while the isogenic strain containing the intronless genome is respiratory competent. Northern analysis indicates that the functional suv3 protein is necessary for the yeast cell in order to process the r1-containing transcripts: in the absence of the suv3 protein the hybridization pattern of the excised r1 intron is altered and the amount of mature 21s rRNA is 50-fold lower. We suggest that the multifunctional suv3 protein, which displays motifs of ATP-dependent RNA helicases, is necessary for the in vivo pathway leading to formation of mature 21s rRNA from the transcripts containing the r1 intron in mitochondria of Saccharomyces cerevisiae.
Similar content being viewed by others
References
Arnberg AC, Van der Horst G, Tabak H (1986) Cell 44:235–242
Bolotin-Fukuhara M, Grivell LA (1992) Ant van Leeuwenhoek 62:131–153
Bos H (1980) PhD thesis, University of Amsterdam
Burke JM (1988) Gene 73:273–294
Butow RA, Zhu H, Perlman P, Conrad-Webb H (1989) Genome 31:757–760
Cech TR (1990) Annu Rev Biochem 59:543–568
Conrad-Webb H, Perlman PS, Zhu H, Butow RA, (1990) Nucleic Acids Res 18:1369–1376
Dujon B (1980) Cell 20:185–197
Garriga G, Bertrand H, Lambowitz AM (1984) Cell 36:623–634
Guthrie C (1991) Science 253:157–163
Jacquier A, Dujon B (1983) Mol Gen Genet 192:487–499
Jacquier A, Dujon B (1985) Cell 41:383–394
Lambowitz AM, Perlman PS (1990) Trends Biochem Sci 15:440–444
Levens D, Ticho B, Ackerman E, Rabinowitz M (1981) J Biol Chem 256:5226–5232
Locker J, Rabinowitz M (1981) Plasmid 6:302–314
Macreadie IG, Scott RM, Zinn AR, Butow RA (1985) Cell 41:395–402
Merten S, Synenki RM, Locker J, Christianson T, Rabinowitz M (1980) Proc Natl Acad Sci USA 77:1417–1421
Morimoto R, Locker J, Synenki RM, Rabinowitz M (1979) J Biol Chem 254:12461–12470
Musters W, Venema J, Van der Linden G, Van Heerikhuizen H, Klootwijk J, Planta RJ (1989) Mol Cell Biol 9:551–559
Rose MD, Winston F, Hieter P (1990) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Ruby SW, Abelson J (1991) Trends Genet 7:79–85
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Seraphin B, Boulet A, Simon M, Faye G (1987) Proc Natl Acad Sci USA 84:6810–6814
Stepien PP, Butow RA (1989) Nucleic Acids Res 18:380
Stepien PP, Margossian SP, Landsman D, Butow RA (1992) Proc Natl Acad Sci USA 89:6813–6817
Tabak HF, Van der Horst G, Osinga KA, Arnberg AC (1984) Cell 39:623–629
Van der Horst G, Tabak HF (1985) Cell 40:759–766
Zhu H, Macreadie IG Butow RA (1987) Mol Cell Biol 7:2530–2537
Zhu H, Conrad-Webb C, Liao XH, Perlman PS, Butow RA (1989) Mol Cell Biol 9:1507–1512
Author information
Authors and Affiliations
Additional information
Communicated by K. Wolf
Rights and permissions
About this article
Cite this article
Stepien, P.P., Kokot, L., Leski, T. et al. The suv3 nuclear gene product is required for the in vivo processing of the yeast mitochondrial 21s rRNA transcripts containing the r1 intron. Curr Genet 27, 234–238 (1995). https://doi.org/10.1007/BF00326154
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00326154