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Phase-contrast microscopy of fresh and cultured pancreatic islet cells of guinea-pigs

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Summary

Isolated cells were prepared from microdissected pancreatic islets of guinea-pigs. Phase-contrast microscopy of the fresh islet cells suspended in a balanced salt solution displayed a number of cellular details, including cytoplasmic secretion granules. There was morphologic evidence of the survival of islet cells in monolayer cultures for up to 3 weeks. A moderate proliferation of cells occurred during the first 2 weeks after explantation. Different types of islet cells could not be distinguished in the phase-contrast microscope.

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Authors and Affiliations

  1. Histological Department, University of Uppsala, Uppsala, Sweden

    Arne Andersson, Claes Hellerström & Birger Petersson

Authors
  1. Arne Andersson
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  2. Claes Hellerström
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  3. Birger Petersson
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Additional information

Research carried out with the financial support of the Swedish Medical Research Council (B 67-12 X-109-03), the Medical Faculty of Uppsala University and the U. S. Public Health Service (Grant AM-05759-05). We thank Assistant Professor Björn Sandström, M. D., for much advice on the tissue-culture method.

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Andersson, A., Hellerström, C. & Petersson, B. Phase-contrast microscopy of fresh and cultured pancreatic islet cells of guinea-pigs. Z. Zellforsch. 82, 110–117 (1967). https://doi.org/10.1007/BF00326104

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  • DOI: https://doi.org/10.1007/BF00326104

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