Summary
A two site immunoradiometric assay (IRMA) for quantification of the recA protein has been recently described (Paoletti et al. 1982). We have used a similar technique to monitor the possible amplification of the ssb protein in E. coli after induction of the SOS repair process by various DNA damaging agents. Under these conditions, while we have been able to detect a full amplification of recA protein, we failed to observed any amplification of the ssb protein.
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References
Baluch J, Chase JW, Sussman R (1980) Synthesis of recA protein and induction of bacteriophage lambda in single-strand deoxyribonucleic acid-binding protein mutants of Escherichia coli. J. Bacteriol 144:480–498
Brandsma JA, Stoorvogel J, Van Sluis CA, Van De Putte P (1982) Effect of lexA and ssb genes, present on a uvrA recombinant plasmid, on the UV survival of Escherichia coli K-12. Gene 18:77–85
Glassberg J, Meyer RR, Kornberg A (1979) Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol 140:14–19
Johnson BJ (1977) Genetic mapping of the lexC113 mutation. Mol Gen Genet 157:91–97
Kirsch HM, Van Houwe G (1976) Stimulation of the synthesis of bacteriophage T4 gene 32 protein by ultraviolet light irradiation. J Mol Biol 108:67–81
Little JW, Mount DW (1982) The SOS regulatory system of Escherichia coli. Cell 29:11–22
McEntee K, Weinstock GM, Lehman IR (1980) RecA proteincatalyzed strand assimilation: stimulation by Escherichia coli single-stranded DNA binding protein. Proc Natl Acad Sci USA 77:857–861
Meyer RR, Glassberg J, Kornberg A (1979) An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication. Proc Natl Acad Sci USA 76:1702–1705
Miles LEM, Lipschitz DA, Bieber CP, Cook JD (1974) Measurement of serum ferritin by a 2-site immunoradiometric assay. Anal Biochem 61:209–224
Moreau PL, Pelico JV, Devoret R (1982) Cleavage of λ repressor and synthesis of recA protein induced by transfered UV-damage F sex factor. Mol Gen Genet 186:170–179
Paoletti C, Salles B, Giacomoni P (1982) An immunoradiometric quantitative assay of Escherichia coli recA protein. Biochimie 64:239–246
Radman M (1975) SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompained by mutagenesis. In: Hanawalt P, Setlow R (eds) Molecular mechanisms for repair of DNA. Plenum Press, New York, p 335–367
Resnick J, Sussman R (1982) Escherichia coli single-strand DNA binding protein from wild type and lexC 113 mutant affect in vitro proteolytic cleavage of phage λ repressor. Proc Natl Acad Sci USA 79:2832–2835
Salles B, Lesca C (1982) Induction of recA protein in Escherichia coli by three platinum(II) compounds. Biochem Biophys Res Commun 105:202–208
Salles B, Paoletti C (in press) Control of UV-induction of recA protein. Proc Natl Acad Sci USA
Sancar A, Williams KR, Chase JW, Rupp WD (1981) Sequence of the ssb gene and protein. Proc Natl Acad Sci USA 78:4274–7278
Shibata T, Das Gupta C, Cunningham RP, Radding CM (1980) Homologous pairing in genetic recombination: formation of D loops by combined action of recA protein and a helix destabilizing protein. Proc Natl Acad Sci USA 77:2606–2610
Vales LD, Chase JW, Murphy JB (1980) Effect of ssbA 1 and lexC113 mutations on lambda prophage induction, bacteriophage growth and cell survival. J Bacteriol 143:887–896
Weiner JH, Bertsch LL, Kornberg A (1975) The desoxyribonucleic acid unwinding protein of Escherichia coli. J Biol Chem 250:1972–1980
West SC, Cassuto E, Howard-Flanders P (1982) Role of ssb protein in recA promoted branch migration reactions. Mol Gen Genet 186:333–338
Whittier RF, Chase JW (1981) DNA repair in E. coli strains deficient in single-strand DNA binding protein. Mol Gen Genet 183:341–347
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Communicated by B.A. Bridges
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Salles, B., Paoletti, C. & Villani, G. Lack of single-strand DNA-binding protein amplification under conditions of SOS induction in E. coli . Molec Gen Genet 189, 175–177 (1983). https://doi.org/10.1007/BF00326074
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DOI: https://doi.org/10.1007/BF00326074