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Uptake and dissimilation of glycerol by wild type and glycerol nonutilizing strains of Neurospora crassa

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Summary

Seven mutant strains defective for utilization of glycerol, glyceraldehyde or dihydroxyacetone were isolated. One strain was deficient for NAD-linked glycerol-3-phosphate dehydrogenase, two for glycerol kinase, and four had no detected enzymatic deficiency, although one of the latter strains was deficient in glycerol uptake. Glycerol uptake was increased by incubation in glycerol, glycerol-3-phosphate, erythritol, and propanediol, and was protein-mediated below 0.14 mM glycerol, but at higher concentrations free diffusion predominated. Glycerol uptake was decreased by cycloheximide and was more sensitive to sodium azide than to iodoacetate.

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Authors and Affiliations

  1. Department of Microbiology, University of Georgia, 30602, Athens, Georgia, USA

    Joseph E. Pyle & H. Branch Howe Jr.

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  1. Joseph E. Pyle
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  2. H. Branch Howe Jr.
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Communicated by F. Kaudewitz

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Pyle, J.E., Howe, H.B. Uptake and dissimilation of glycerol by wild type and glycerol nonutilizing strains of Neurospora crassa . Molec Gen Genet 189, 166–168 (1983). https://doi.org/10.1007/BF00326071

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  • DOI: https://doi.org/10.1007/BF00326071

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