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Plasmid transformation in Bacillus subtilis: Symmetry of gene conversion in transformation with a hybrid plasmid containing chromosomal DNA

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Summary

Allele transfer (“conversion”) was analyzed in transformations with a CmR determining hybrid plasmid, which contained a chromosomal gene controlling threonine prototrophy. In transformations, where a thr +-cell was transformed with the thr - plasmid, the chromosomal allele was efficiently converted to the plasmid genotype. This process of gene conversion was rec dependent and greatly enhanced when monomeric rather than unfractionated plasmid DNA was used.

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Authors and Affiliations

  1. Abt. Trautner, Max-Planck-Institut für molekulare Genetik, Ihnestraße 63/73, D-1000, Berlin 33

    A. Iglesias & T. A. Trautner

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  1. A. Iglesias
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  2. T. A. Trautner
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Communicated by E. Bautz

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Iglesias, A., Trautner, T.A. Plasmid transformation in Bacillus subtilis: Symmetry of gene conversion in transformation with a hybrid plasmid containing chromosomal DNA. Molec Gen Genet 189, 73–76 (1983). https://doi.org/10.1007/BF00326057

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  • DOI: https://doi.org/10.1007/BF00326057

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