Summary
The gene of B. subtilis phage SPR, specifying DNA methyltransferase activity was identified within a 3.1 Md EcoRI fragment of SPR DNA. This fragment was cloned in E. coli using a λ insertion vector. A 1.25 Md PstI fragment with this gene was subsequently derived from the λ/SPR hybrid phage and subcloned in B. subtilis using a new SPP1 vector, whose construction is described.
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Communicated by E. Bautz
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Behrens, B., Pawlek, B., Morelli, G. et al. Restriction and modification in Bacillus subtilis: Construction of hybrid λ and SPP1 phages containing a DNA methyltransferase gene from B. subtilis phage SPR. Molec Gen Genet 189, 10–16 (1983). https://doi.org/10.1007/BF00326048
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DOI: https://doi.org/10.1007/BF00326048