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Physical mappings of chloroplast DNA from liverwort Marchantia polymorpha L. cell suspension cultures

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Summary

Physical maps of liverwort Marchantia polymorpha chloroplast DNA were constructed with restriction endonucleases, BamHI, SmaI, KpnI, and XhoI. The molecular size, calculated from the sum of the restriction fragments, is 121.0±1.0 kilobase (kb) pairs. This value is in good agreement with that of 118.7±2.0 kb pairs obtained from contour length measurements of the chloroplast DNA electron micrographs. The physical map indicates that the chloroplast DNA contains two copies of inverted repeats. The length of the inverted repeat is at most 11.7 kb pairs. Southern hybridization analysis indicates that two sets of the chloroplast ribosomal RNA genes are located in the inverted repeat regions. The site of the ribulose bisphosphate (RuBP) carboxylase (large subunit) gene was also determined on a BamHI fragment, Ba5, by using 32P-labeled DNA fragments containing the tobacco chloroplast RuBP carboxylase gene as a probe.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan

    Kanji Ohyama, Yoshiaki Yamano, Hideya Fukuzawa & Tohru Komano

  2. Department of Biophysics, Faculty of Science, Kyoto University, 606, Kyoto, Japan

    Hideo Yamagishi & Sinji Fujimoto

  3. National Institute of Genetics, 411, Mishima, Shizuoka-ken, Japan

    Masahiro Sugiura

Authors
  1. Kanji Ohyama
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  2. Yoshiaki Yamano
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  3. Hideya Fukuzawa
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  4. Tohru Komano
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  5. Hideo Yamagishi
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  6. Sinji Fujimoto
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  7. Masahiro Sugiura
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Communicated by J. Schell

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Ohyama, K., Yamano, Y., Fukuzawa, H. et al. Physical mappings of chloroplast DNA from liverwort Marchantia polymorpha L. cell suspension cultures. Molec Gen Genet 189, 1–9 (1983). https://doi.org/10.1007/BF00326047

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  • DOI: https://doi.org/10.1007/BF00326047

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