Summary
DNA sequences of Candida utilis capable of supporting autonomous replication of plasmids in Saccharomyces cerevisiae were isolated by cloning HindIII restriction fragments of total DNA of C. utilis in the cosmid pHC79-arg4-43 carrying the gene of argininosuccinate-lyase as the selective marker for yeast transformation. Three hybrid plasmids replicating in S. cerevisiae were obtained and growth characteristics of plasmid-carrying cells were investigated. The hybridization experiments show that all hybrid plasmids obtained contain inserts of C. utilis DNA, all of mitochondrial origin. There was no hybridization to S. cerevisiae total DNA observed. One of the hybrid plasmids (pESC17) was characterized in detail. Localization of the replication origin of the cloned fragment was accomplished by recloning of subfragments in the plasmid pHC79-arg4-43. It was shown that effective replication in the yeast cells necessitates the presence of a 300 bp sequence. A primary structure of this DNA fragment showed an extremely high AT content and the absence of GC blocks.
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Tikhomirova, L.P., Kryukov, V.M., Strizhov, N.I. et al. mtDNA sequences of Candida utilis capable of supporting autonomous replication of plasmids in Saccharomyces cerevisiae . Molec. Gen. Genet. 189, 479–484 (1983). https://doi.org/10.1007/BF00325913
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DOI: https://doi.org/10.1007/BF00325913