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Kasugamycin resistant mutants of Bacillus stearothermophilus lacking the enzyme for the methylation of two adjacent adenosines in 16S ribosomal RNA

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Summary

Several mutants of B. stearothermophilus have been isolated that are resistant to the antibiotic kasugamycin. One of these is shown to lack dimethylation of two adjacent adenosines in the 16S ribosomal RNA. All mutants that were analyzed biochemically lack the enzyme that is able to methylate this site. Ribosomal sensitivity and resistance to kasugamycin in B. stearothermophilus is therefore, like in E. coli, closely connected with dimethylation of the adenosines.

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Abbreviations

ksg:

kasugamycin

SAM:

S-adenosyl-l-methionine

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Authors and Affiliations

  1. Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands

    C. P. J. J. Van Buul, J. B. L. Damm & P. H. Van Knippenberg

Authors
  1. C. P. J. J. Van Buul
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  2. J. B. L. Damm
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  3. P. H. Van Knippenberg
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Communicated by K. Isono

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Van Buul, C.P.J.J., Damm, J.B.L. & Van Knippenberg, P.H. Kasugamycin resistant mutants of Bacillus stearothermophilus lacking the enzyme for the methylation of two adjacent adenosines in 16S ribosomal RNA. Molec. Gen. Genet. 189, 475–478 (1983). https://doi.org/10.1007/BF00325912

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  • DOI: https://doi.org/10.1007/BF00325912

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