Summary
The regulation of synthesis of arg enzymes in Salmonella typhimurium by the arginine repressor of Escherichia coli K-12 has been reevaluated using a strain of S. typhimurium in which the argR gene was rendered nonfunctional by inserting the translocatable tetracyclineresistance element Tn10 into the argR gene. In contrast to previous studies, the introduction of the argR + allelle of E. coli on an F-prime factor to the argR::Tn10 S. typhimurium strain reduced the synthesis of arg enzymes to essentially wild-type levels. The elevated levels of arg enzymes observed in other hybrid merodiploids may have been the consequence of the formation of hybrid repressor molecules. The readily scoreable phenotype of tetracycline resistance facilitated establishing linkage of cod and argR (0.6% cotransduction) by P22 phage-mediated transduction.
Similar content being viewed by others
References
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44:1–56
Blazy DL, Burns RO (1980) Gene ilv Y of Salmonella typhimurium. J Bacteriol 142:1015–1018
Bochner BR, Huan H-C, Schieven GL, Ames BN (1980) Positive selection for loss of tetracycline resistance. J Bacteriol 143:926–933
Bollon AP, Vogel HJ (1973) Regulation of argE-argH expression with arginine derivatives in Escherichia coli: extreme non-uniformity of repression and conditional repressive action. J Bacteriol 114:632–640
Fuchs RL, Madonna MJ, Brenchley JE (1982) Identification of the structural genes for glutamate synthase and genetic characterization of this region of the Salmonella typhimurium chromosome. J Bacteriol 149:906–915
Itikawa H, Baumberg S, Vogel HJ (1968) Enzymic basis for a genetic suppression: accumulation and deacylation of N-acetylglutamic-γ-semialdehyde in enterobacterial mutants. Biochim Biophys Acta 159:547–550
Jacoby G (1972) Control of the argECBH cluster in Escherichia coli. Mol Gen Genet 117:337–348
Jobanputra RS, Datta N (1974) Trimethoprim R factors in enterobacteria from clinical specimens. J Med Microbiol 1:169–177
Johnson HM, Barnes WM, Chumley FG, Bossi L, Roth JR (1980) Model for regulation of the histidine operon of Salmonella. Proc Natl Acad Sci USA 77:508–512
Kadner RJ, Maas WK (1971) Regulatory gene mutations affecting arginine biosynthesis in Escherichia coli. Mol Gen Genet 111:1–14
Kelln RA, Foltermann KF, O'Donovan GA (1975a) Location of the argR gene on the chromosome of S. typhimurium. Mol Gen Genet 139:277–284
Kelln RA, Kinahan JJ, Foltermann KF, O'Donovan GA (1975b) Pyrimidine biosynthetic enzymes of Salmonella typhimurium repressed specifically by growth in the presence of cytidine. J Bacteriol 124:764–774
Kleckner N, Chan PK, Tye B-K, Bostein D (1975) Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol 97:561–575
Loper JC, Adams E (1965) Purification and properties of histidinol dehydrogenase from Salmonella typhimurium. J Biol Chem 240:788–795
Manson MD, Yanofsky C (1976) Tryptophan operon regulation in interspecific hybrids of enteric bacteria. J Bacteriol 126:679–689
Meyer RJ, Shapiro JA (1980) Genetic organization of the broadhost-range IncP-1 plasmid R751. J Bacteriol 143:1362–1373
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
Nakamura Y (1978) RNA polymerase mutant with altered sigma factor in Escherichia coli. Mol Gen Genet 165:1–6
Olsen RH, Siak JS, Gray RH (1974) Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. J Virol 14:689–699
Prescott LM, Jones ME (1969) Modified methods for the determination of carbamyl aspartate. Anal Biochem 32:408–419
Roth JR (1970) Genetic techniques in studies of bacterial metabolism. Meth Enzymol 27A:3–35
Sanderson KE, Hartman PE (1978) Linkage map of Salmonella typhimurium, edition V. Microbiol Rev 42:471–519
Vogel HJ, Vogel RH (1974) Enzymes of arginine biosynthesis and their repressive control. Adv Enzymol 40:65–90
Wessler SR, Calvo JM (1981) Control of leu operon expression in Escherichia coli by a transcription attenuation mechanism. J Mol Biol 149:579–597
Author information
Authors and Affiliations
Additional information
Communicated by G.A. O'Donovan
Rights and permissions
About this article
Cite this article
Gardner, M.M., Hennig, D.O. & Kelln, R.A. Control of arg gene expression in salmonella typhimurium by the arginine repressor from Escherichia coli K-12. Molec. Gen. Genet. 189, 458–462 (1983). https://doi.org/10.1007/BF00325909
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00325909