Summary
In somatic hybrids of each of four chlorate-resistant cell lines of Nicotiana tabacum L., two of which were also nitrate-nonutilizing, and a common chlorate-sensitive parent, nitrate utilization and/or chlorate resistance were found to be recessive traits. Complementation for nitrate utilization was observed in somatic hybrids of the two nitrate-nonutilizing cell lines. No chlorate-resistant or nitrate-nonutilizing seedlings were detected among a large number of progeny resulting from the self-fertilization of a somatic hybrid plant one of whose parents was one of the nitrate-nonutilizing cell lines.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ashton NW, Grimsley NH, Cove DJ (1979) Analysis of gametophytic development of the moss, Physcomitrella patens, using auxin or cytokinin resistant mutants. Planta 144:427–435
Aviv D, Fluhr R, Edelman M, Galun E (1980) Progeny analysis of the interspecific somatic hybrids Nicotiana tabacum (CMS)+ Nicotiana sylvestris with respect to nuclear and chloroplast markers. Theor Appl Genet 56:145–150
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403
Belliard G, Pelletier G, Vedel F, Quertier F (1978) Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum. Mol Gen Genet 165:231–237
Evola SV (1983) Chlorate-resistant variants of Nicotiana tabacum L. I. Selection in vitro and phenotypic characterization of cell lines and regenerated plants. Mol Gen Genet 189:447–454
Evola SV, Earle ED, Chaleff RS (1983) The use of genetic markers selected in vitro for the isolation and genetic verification of intraspecific somatic hybrids of Nicotiana tabacum L. Mol Gen Genet 189:441–446
Gleba Yu Yu, Piven NM, Komarnitskii IK, Sytnik KM (1978) Parasexual cytoplasmic Nicotiana tabacum + N. debneyi hybrids (cybrids), obtained by protoplast fusion. Akad Nauk SSSR Doklady 240:225–227
Glimelius K, Eriksson T, Grafe R, Müller AJ (1978) Somatic hybridization of nitrate reductase-deficient mutants of Nicotiana tabacum by protoplast fusion. Physiol Plant 44:273–277
Grimsley NH, Ashton NW, Cove DJ (1977a) Complementation analysis of auxotrophic mutants of the moss, Physcomitrella patens, using protoplast fusion. Mol Gen Genet 155:103–107
Grimsley NH, Ashton NW, Cove DJ (1977b) The production of somatic hybrids by protoplast fusion in the moss, Physcomitrella patens. Mol Gen Genet 154:97–100
Marton L, Sidorov V, Biasini G, Maliga P (1982) Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia. Mol Gen Genet 187:1–3
Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance: chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris and isolation of their somatic hybrids. Mol Gen Genet 179:693–698
Muller HJ (1923) A simple formula giving the number of individuals required for obtaining one of a given frequency. Am Nat 57:66–73
Scowcroft W, Larkin P (1981) Chloroplast DNA assorts randomly in intraspecific hybrids of Nicotiana debneyi. Theor Appl Genet 60:179–184
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Rights and permissions
About this article
Cite this article
Evola, S.V. Chlorate-resistant variants of Nicotiana tabacum L.. Molec. Gen. Genet. 189, 455–457 (1983). https://doi.org/10.1007/BF00325908
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00325908