Skip to main content
SpringerLink
Log in

Chlorate-resistant variants of Nicotiana tabacum L.

I. Selection in vitro and phenotypic characterization of cell lines and regenerated plants

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Four chlorate-resistant cell lines were selected from cells derived from mesophyll tissue of a dihaploid plant of Nicotiana tabacum L. Two of those cell lines, clr0 and clr19, have a high level of resistance to chlorate, cannot assimilate nitrate and lack NADH-nitrate reductase activity. The other two cell lines, clr15 and clr30, have a lower level of resistance to chlorate, can assimilate nitrate and have an altered level and regulation of NADH-nitrate reductase activity. NADH-nitrate reductase activity was partially restored in mixed extracts of clr0 and clr19 cells. Both clr0 and clr19 cells take up nitrate from the medium. Cells of the clr0 line but not those of the clr19 line lack xanthine oxidase activity. Plants regenerated from each of the cell lines, as well as secondary callus cultures initiated from those plants, exhibit the same phenotype as the primary cell lines with respect to chlorate resistance and nitrate utilization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Behrend J, Mateles RI (1976) Nitrogen metabolism in plant cell suspension cultures. II. Role of organic acids during growth on ammonia, Plant Physiol 58:510–512

    Google Scholar 

  • Cataldo DA, Haroon M, Schrader LE, Youngs VL (1975) Rapid colorimetric determination of nitrate in plant tissues by nitration of salicylic acid. Commun Soil Sci Plant Analysis 6:71–80

    Google Scholar 

  • Chaleff RS, Parsons MF (1978a) Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum. Proc Natl Acad Sci USA 75:5104–5107

    Google Scholar 

  • Chaleff RS, Parsons MF (1978b) Isolation of a glycerol-utilizing mutant of Nicotiana tabacum. Genetics 89:723–728

    Google Scholar 

  • Cove DJ (1976a) Chlorate toxicity in Aspergillus nidulans. Mol Gen Genet 146:147–159

    Google Scholar 

  • Cove DJ (1976b) Chlorate toxicity in Aspergillus nidulans: the selection and characterization of chlorate resistant mutants. Heredity 36:191–203

    Google Scholar 

  • Cove DJ (1979) Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev 54:291–327

    Google Scholar 

  • Evola SV, Earle ED, Chaleff RS (1983) The use of genetic markers selected in vitro for the isolation and genetic verification of intraspecific somatic hybrids of Nicotiana tabacum L Mol Gen Genet 189:441–446

    Google Scholar 

  • Feenstra WJ, Jacobsen E (1981) Isolation of a nitrate reductase deficient mutant of Pisum sativum by means of selection for chlorate resistance. Theor Appl Genet 58:39–42

    Google Scholar 

  • Gamborg OL, Shyluk JP (1970) The culture of plant cells with ammonium salts as the sole nitrogen source. Plant Physiol 45:598–600

    Google Scholar 

  • Glaser JJ, DeMoss JA (1972) Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol Gen Genet 116:1–10

    Google Scholar 

  • Heimer YM, Wray JL, Filner P (1969) The effect of tungstate on nitrate assimilation in higher plant tissues. Plant Physiol 44:1197–1199

    Google Scholar 

  • Hince T, Neale S (1974) A comparison of the mutagenic action of the methyl and ethyl derivatives of nitrosamides and nitrosamidines on Escherichia coli. Mutat Resia 24:383–387

    Google Scholar 

  • Horsch RB, King J, Jones GE (1980) Measurement of cultured plant cell growth on filter paper discs. Can J Bot 58:2402–2406

    Google Scholar 

  • King J, Khanna V (1980) A nitrate reductase-less variant isolated from suspension cultures of Datura innoxia (Mill.). Plant Physiol 66:632–636

    Google Scholar 

  • Kinghorn JR, Pateman JA (1975) Studies of partially repressed mutants at the tamA and areA loci in Aspergillus nidulans. Mol Gen Genet 140:137–147

    Google Scholar 

  • Kleinhofs A, Warner RL, Muehlbauer FJ, Nilan RA (1978) Induction and selection of specific gene mutations in Hordeum and Pisum. Mutat Res 51:29–35

    Google Scholar 

  • Lewis CM, Fincham JRS (1970) Genetics of nitrate reductase in Ustilago maydis. Genet Res (Cambr) 16:151–163

    Google Scholar 

  • MacGregor CH (1975) Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli. J Bacteriol 121:1117–1121

    Google Scholar 

  • Marton L, Dung TM, Mendel RR, Maliga P (1982) Nitrate reductase deficient cell lines from haploid protoplast cultures of Nicotiana plumbaginifolia. Mol Gen Genet 182:301–304

    Google Scholar 

  • Massey V (1959) The microestimation of succinate and the extinction coefficient of cytochrome c. Biochim Biophys Acta 34:225–261

    Google Scholar 

  • Mendel R (1980) Comparative affinity chromatography of nitrate reductase from wild-type and molybdenum cofactor-defective cell cultures of Nicotiana tabacum. Biochem Physiol Pflanzen 175:216–227

    Google Scholar 

  • Mendel R, Alikulov AA, Lvov NP, Müller AJ (1981) Presence of the molybdenum-cofactor in nitrate reductase-deficient mutant cell lines of Nicotiana tabacum. Mol Gen Genet 181:395–399

    Google Scholar 

  • Mendel RR, Müller AJ (1976) A common genetic determinant of xanthine dehydrogenase and nitrate reductase in Nicotiana tabacum. Biochem Physiol Pflanzen 170S:538–541

    Google Scholar 

  • Mendel RR, Müller AJ (1978) Reconstitution of NADH-nitrate reductase in vitro from nitrate reductase-deficient Nicotiana tabacum mutants. Mol Gen Genet 161:77–80

    Google Scholar 

  • Mendel RR, Müller AJ (1979) Nitrate reductase deficient mutant cell lines of Nicotiana tabacum. Further biochemical characterization. Mol Gen Genet 177:145–153

    Google Scholar 

  • Mendel RR, Müller AJ (1980) Comparative characterization of nitrate reductase from wild-type and molybdenum cofactor defective cell cultures of Nicotiana tabacum. Plant Sci Lett 18:277–288

    Google Scholar 

  • Müller AJ, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161:67–76

    Google Scholar 

  • Murphy TM, Imbrie CW (1981) Induction and characterization of chlorate-resistant strains of Rosa damascena cultured cells. Plant Physiol 67:910–916

    Google Scholar 

  • Neale S (1972) Effect of pH and temperature on nitrosamide-induced mutation in Escherichia coli. Mutat Res 14:155–164

    Google Scholar 

  • Nichols GL, Syrett PJ (1978) Nitrate reductase deficient mutants of Chlamydomonas reinhardii: isolation and genetics. J Gen Microbiol 108:71–78

    Google Scholar 

  • Oostindier-Braaksma FJ, Feenstra WJ (1973) Isolation and characterization of chlorate-resistant mutants of Arabidopsis thaliana. Mutat Res 19:175–185

    Google Scholar 

  • Poulik MD (1957) Starch gel electrophoresis in a discontinuous system of buffers. Nature 180:1477–1479

    Google Scholar 

  • Sosa FM, Ortega T, Barea JL (1978) Mutants from Chlamydomonas reinhardii affected in their nitrate assimilation capability. Plant Sci Lett 11:51–59

    Google Scholar 

  • Strauss A, Bucher F, King PJ (1981) Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus I. A NO3 - non-utilizing clone. Planta 153:75–80

    Google Scholar 

  • Thomas RJ, Schrader LE (1981) Ureide metabolism in higher plants. Phytochemistry 20:361–371

    Google Scholar 

  • Tomsett AB, Garrett RH (1980) The isolation and characterization of mutants defective in nitrate assimilation in Neurospora crassa. Genetics 95:649–660

    Google Scholar 

Download references

Author information

Author notes

  1. Stephen V. Evola

    Present address: Biology Department, Brookhaven National Laboratory, 11973, Upton, NY, USA

Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY, USA

    Stephen V. Evola

Authors
  1. Stephen V. Evola
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Melchers

Rights and permissions

Reprints and permissions

About this article

Cite this article

Evola, S.V. Chlorate-resistant variants of Nicotiana tabacum L.. Molec. Gen. Genet. 189, 447–454 (1983). https://doi.org/10.1007/BF00325907

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00325907

Keywords

Search

Navigation