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Complementation analysis of a nitrate reductase deficient Hyoscyamus muticus cell line by somatic hybridisation

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Summary

Complementation of a nitrate reductase deficient variant of Hyoscyamus muticus (MA-2) and nitrate reductase apoenzyme (nia-115) and cofactor mutants (cnx-68) of Nicotiana tabacum was studied by protoplast fusion. Selection of prototrophic intergeneric somatic hybrids was achieved in combination of MA-2 with the apoenzyme mutant nia-115 of N. tabacum. The H. muticus MA-2 line was therefore classified to be a cnx type variant possessing an altered molybdenum cofactor of the nitrate reductase enzyme complex but unaffected in the apoprotein of nitrate reductase. The nitrate reductase deficient and chlorate resistant characters of MA-2 were functionally coupled recessive traits. Nitrate reductase activity accompanied by chlorate sensitivity could be detected only under inductive conditions in the somatic hybrids. The inductive expression of nitrate reductase in the somatic hybrids arising from the combination of cells harbouring either the inductive or constitutive type nitrate reductase is discussed.

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Abbreviations

DTT:

1,4-Dithio-DL-threitol

Mo-co:

molybdenum containing cofactor

PEG:

polyethylene glycol

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Author notes

  1. G. B. Lázár

    Present address: Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

Authors and Affiliations

  1. Friedrich Miescher Institut, P.O. Box 2543, CH-4002, Basel, Switzerland

    G. B. Lázár, H. Fankhauser & I. Potrykus

Authors
  1. G. B. Lázár
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  2. H. Fankhauser
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  3. I. Potrykus
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Communicated by G. Melchers

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Lázár, G.B., Fankhauser, H. & Potrykus, I. Complementation analysis of a nitrate reductase deficient Hyoscyamus muticus cell line by somatic hybridisation. Molec. Gen. Genet. 189, 359–364 (1983). https://doi.org/10.1007/BF00325895

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  • DOI: https://doi.org/10.1007/BF00325895

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