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The ribosomal protein gene cluster of Mycoplasma capricolum

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Summary

The DNA sequence of the part of the Mycoplasma capricolum genome that contains the genes for 20 ribosomal proteins and two other proteins has been determined. The organization of the gene cluster is essentially the same as that in the S10 and spc operons of Escherichia coli. The deduced amino acid sequence of each protein is also well conserved in the two bacteria. The G+C content of the M. capricolum genes is 29%, which is much lower than that of E. coli (51%). The codon usage pattern of M. capricolum is different from that of E. coli and extremely biased to use of A and U(T): about 91% of codons have A or U in the third position. UGA, which is a stop codon in the “universal” code, is used more abundantly than UGG to dictate tryptophan.

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References

  • Brunes M, Shumann R, Wittinghofer F (1985) Cloning and sequencing of adenylate kinase gene (adk) of Escherichia coli. Nucleic Acids Res 19:7139–7151

    Google Scholar 

  • Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M (1983) The spc ribosomal protein operon of Escherichia coli: Sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res 9:2599–2616

    Google Scholar 

  • Chen EY, Seeburg PH (1985) Supercoil sequencing: A fast and simple method for sequencing plasmid DNA. DNA 4:165–170

    Google Scholar 

  • Higo K, Otaka E, Osawa S (1982) Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: Correlation to Escherichia coli 30S proteins. Mol Gen Genet 185:239–244

    Google Scholar 

  • Iwami M, Muto A, Yamao F, Osawa S (1984) Nucleotide sequence of the rrnB 16S ribosomal RNA gene from Mycoplasma capricolum. Mol Gen Genet 196:317–322

    Google Scholar 

  • Jukes TH (1985) A change of genetic code in Mycoplasma capricolum. J Mol Evol 22:361–362

    Google Scholar 

  • Jukes TH, Bhushan V (1986) Silent nucleotide substitutions and G+C content of some mitochondrial and bacterial genes. J Mol Evol 24:39–44

    Google Scholar 

  • Kawauchi Y, Muto A, Osawa S (1982) The protein composition of Mycoplasma capricolum. Mol Gen Genet 188:7–11

    Google Scholar 

  • Kawauchi Y, Muto A, Osawa S (1984) Molecular cloning of ribosomal protein genes from Mycoplasma capricolum. Mol Gen Genet 196:521–525

    Google Scholar 

  • Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, Cambridge

    Google Scholar 

  • Korneluk RGF, Quan F, Gravel RA (1985) Rapid and reliable sequencing of double-stranded DNA. Gene 40:317–323

    Google Scholar 

  • Lindahl L, Zengel JM (1986) Ribosomal genes in Escherichia coli. Annu Rev Genet 20:297–326

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, New York, USA

    Google Scholar 

  • Messing J, Crea R, Seeburg PH (1981) A system for shutgun DNA sequencing. Nucleic Acids Res 9:309–321

    Google Scholar 

  • Muto A, Osawa S (1987) The guanine and cytosine content of genomic DNA and bacterial evolution. Proc Natl Acad Sci USA 84:166–169

    Google Scholar 

  • Muto A, Kawauchi Y, Yamao F, Osawa S (1984) Preferential use of A-and U-rich codons for Mycoplasma capricolum ribosomal proteins S8 and L6. Nucleic Acids Res 12:8209–8219

    Google Scholar 

  • Muto A, Yamao F, Kawauchi Y, Osawa S (1985) Codon usage in Mycoplasma capricolum. Proc Jpn Acad [B] 61:12–15

    Google Scholar 

  • Muto A, Yamao F, Osawa S (1987) The genome of Mycoplasma capricolum. Prog Nucleic Acid Res Mol Biol 34:29–58

    Google Scholar 

  • Nomura M, Gourse R, Baughman G (1984) Regulation of the synthesis of ribosomes and ribosomal components. Annu Rev Biochem 53:75–118

    Google Scholar 

  • Ohama T, Yamao F, Muto A, Osawa S (1987) Organization and codon usage of streptomycin-operon in high genomic G+C bacterium Micrococcus luteus. J Bacteriol 169: in press

  • Razin S (1985) Molecular biology and genetics of mycoplasma (Mollicutes). Microbiol Rev 49:419–455

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Sawada M, Muto A, Iwami M, Yamao F, Osawa S (1984) Organization of ribosomal RNA genes in Mycoplasma capricolum. Mol Gen Genet 196:311–316

    Google Scholar 

  • Sueoka N (1961) Correlation between base composition of deoxyribonucleic acid and amino acid composition of protein. Proc Natl Acad Sci USA 47:1141–1149

    Google Scholar 

  • Yamao F, Muto A, Kawauchi Y, Iwami M, Iwagami S, Azumi Y, Osawa S (1985) UGA is read as tryptophan in Mycoplasma capricolum. Proc Natl Acad Sci USA 82:2306–2309

    Google Scholar 

  • Yanish-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119

    Google Scholar 

  • Zurawski G, Zurawski SM (1985) Structure of the Escherichia coli S10 ribosomal protein operon. Nucleic Acids Res 13:4521–4526

    Google Scholar 

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Communicated by K. Isono

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Ohkubo, S., Muto, A., Kawauchi, Y. et al. The ribosomal protein gene cluster of Mycoplasma capricolum . Molec Gen Genet 210, 314–322 (1987). https://doi.org/10.1007/BF00325700

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