Skip to main content
SpringerLink
Log in

Influence of temperature and short-term storage on the stability of norepinephrine in plasma samples

  • Short Communications
  • Published:
Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

Catecholamines, as endogenous substances, are subject to biological degradation. The conditions of plasma handling for the determination of norepinephrine were investigated by using plasma samples of patients and control subjects with special regard to temperature and duration of storage. Previous studies have reported a remarkable i.v. stability of plasma norepinephrine for several hours. Here these findings were supported in a series of plasma samples which were kept either at 0° C or at room temperature. Another series of samples, which had been frozen immediately after collection, stored and rethawed, showed, however, great losses in norepinephrine within 1 to 2 h upon standing. This degradation of norepinephrine could not be attributed to monomaine oxidase (MAO) activity, because the addition of the MAO inhibitor pargyline was found to have no influence on the stability of norepinephrine. Recommendations for handling and storing of plasma samples for norepinephrine determinations are given.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Carruthers M, Taggart P, Conway N, Bates D, Somerville W (1970) Lancet 2:62–67

    Google Scholar 

  2. Pettersson J, Hussi E, Jänne J (1980) Scand J Clin Invest 40:297–303

    Google Scholar 

  3. Falconer AD, Lake D, MacDonald IA (1982) J Neurosci Meth 6:261–271

    Google Scholar 

  4. Goldstein DS (1986) In: Krstulovic AM (ed) Quantitative analysis of catecholamines and related compounds Wiley, New York, p 126f

    Google Scholar 

  5. Smedes F, Kraak JC, Poppe H (1982) J Chromatogr 231:25–39

    Google Scholar 

  6. Kringe K, Greb H, Bayha G, Kranich S, Mitrovic V (1986) LABO 22:7–12

    Google Scholar 

  7. Volz M, Husseini H, Mitrovic V, Gottschalk G, Schürer H, Schlepper M (1992) LABO 1–2:32–38

    Google Scholar 

  8. Powis G (1975) Biochem Pharmacol 24:707–712

    Google Scholar 

  9. McCullough H (1968) J Clin Pathol 21:759–763

    Google Scholar 

  10. Jarman J, Glover V, Sandler M, Turjanski M, Stern G (1993) J Neural Transm 5:1–4

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Klinische Pharmakologie, Max Planck Institute for Clinical and Physiological Research, Kerckhoff-Klinik, Benekestrasse 2-8, D-61231, Bad Nauheim, Germany

    Manfred Volz, Gisela Gottschalk, Hussein Husseini, Veselin Mitrovic & Martin Schlepper

Authors
  1. Manfred Volz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gisela Gottschalk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hussein Husseini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Veselin Mitrovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Martin Schlepper
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Volz, M., Gottschalk, G., Husseini, H. et al. Influence of temperature and short-term storage on the stability of norepinephrine in plasma samples. Fresenius J Anal Chem 348, 696–698 (1994). https://doi.org/10.1007/BF00325577

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00325577

Keywords

Search

Navigation