Summary
In the present work, the effects of drugs on the storage, uptake and synthesis of catecholamines in intrinsic and extrinsic adrenergic neurones of the guinea-pig intestine are compared, using the fluorescence histochemical technique for localising catecholamines. In respect to the properties examined in this work, the intrinsic adrenergic neurones of the proximal colon of the guinea-pig were found to be qualitatively similar to adrenergic neurones of the sympathetic chains: the intrinsic cells and their terminals are depleted by reserpine or guanethidine; they concentrate and retain catecholamines and this uptake is blocked by desmethylimipramine or phenoxybenzamine; after depletion by reserpine, the fluorescence can be restored by the dopamine and noradrenaline precursor, dopa and this restoration is prevented by blocking the decarboxylation of dopa to dopamine. However, there are clear quantitative differences: the terminals of intrinsic neurones are less susceptible than are extrinsic neurones to depletion by reserpine, guanethidine or 6-hydroxydopamine; the intrinsic neurones more readily retain noradrenaline after reserpinisation. It is suggested that quantitative differences between extrinsic and intrinsic neurones of the intestine could involve a difference in the activity of monoamine oxidase.
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Axelrod, J., Hertting, G., Potter, L. T.: Effect of drugs on the uptake and release of H3-norepinephrine in the rat heart. Nature (Lond.) 194, 297 (1962).
—, Whitby, L. G., Hertting, G.: Effect of psychotropic drugs on the uptake of H3-norepinephrine by tissues. Science 133, 383–384 (1961).
Bartholini, G., Pletscher, A.: Cerebral accumulation and metabolism of C14-dopa after selective inhibition of peripheral decarboxylase. J. Pharmacol. exp. Ther. 161, 14–20 (1968).
Burkard, W. P., Gey, K. F., Pletscher, A.: Inhibition of decarboxylase of aromatic amino acids by 2,3,4-trihydroxybenzylhydrazine and its seryl derivatives. Arch. Biochem. 107, 187–196 (1964).
Cass, R., Kuntzman, R., Brodie, B. B.: Norepinephrine depletion as a possible mechanism of action of guanethidine (SU 5864), a new hypotensive agent. Proc. Soc. exp. Biol. (N. Y.) 103, 871–872 (1960).
Chang, C. C., Costa, E., Brodie, B. B.: Interaction of guanethidine with adrenergic neurons. J. Pharmacol. exp. Ther. 147, 303–312 (1965).
Corrodi, H., Fuxe, K., Hamberger, B., Ljungdahl, A.: Studies on central and peripheral noradrenaline neurons using a new dopamine-β-hydroxylase inhibitor. Europ. J. Pharmacol. 12, 145–155 (1970).
Costa, M., Furness, J. B., Gabella, G.: Catecholamine containing nerve cells in the mammalian myenteric plexus. Histochemie 25, 103–106 (1971).
- Gabella, G.: Adrenergic innervation of the alimentary canal. Z. Zellforsch. (in press) (1971).
Ehinger, B., Falck, B.: Uptake of some catecholamines and their precursors into neurons of the rat ciliary ganglion. Acta physiol. scand. 78, 132–141 (1970).
Euler, U. S. von, Lishajko, F.: Effect of reserpine on the uptake of catecholamines in isolated nerve storage granules. Int. J. Neuropharmacol. 2, 127–134 (1963).
Furness, J. B.: An electrophysiological study of the innervation of the smooth muscle of the colon. J. Physiol. (Lond.) 205, 549–562 (1969).
—: The origin and distribution of adrenergic nerve fibers in the guinea-pig colon. Histochemie 21, 295–306 (1970).
—, Costa, M.: Morphology and distribution of intrinsic adrenergic neurones in the proximal colon of the guinea-pig. Z. Zellforsch. 220, 346–363 (1971).
Gabella, G., Costa, M.: itLe fibre adrenergiche nel canale alimentare. G. Accad. Med. Torino 130, 1–12 (1967).
Hamberger, B.: Reserpine resistant uptake of catecholamines in isolated tissues of the rat. A histochemical study. Acta physiol. scand. 71, Suppl. 295, 1–56 (1967).
Laverty, R., Sharman, D. F., Vogt, M.: Action of 2,4,5-trihydroxyphenylethylamine on the storage and release of noradrenaline. Brit. J. Pharmacol. 24, 549–560 (1965).
Lundborg, P.: Studies on the uptake and subcellular distribution of catecholamines and their α-methyiated analogues. Acta physiol. scand. 72, Suppl. 302, 1–34 (1967).
—, Stitzel, R. E.: Studies on the dual action of guanethidine in sympathetic nerves. Acta physiol. scand. 72, 100–107 (1968).
Maitre, L.: Presence of α-methyl-dopa metabolites in heart and brain of guinea-pigs treated with α-methyl-tyrosine. Life Sci. 4, 2249–2256 (1965).
Malmfors, T., Sachs, Ch.: Degeneration of adrenergic nerves produced by 6-hydroxydopamine. Europ. J. Pharmacol. 3, 89–92 (1968).
Muscholl, E., Vogt, M.: The action of reserpine on the peripheral sympathetic system. J. Physiol. (Lond.) 141, 132–155 (1958).
Nilsson, O.: Rate of depletion of noradrenaline in some peripheral tissue induced by a reserpine injection. Experientia (Basel) 20, 679 (1964).
Norberg, K.-A.: Drug induced changes in monoamine levels in the sympathetic adrenergic ganglion cells and terminals. A histochemical study. Acta physiol. scand. 65, 221–234 (1965).
Orden, L. S. van, Schaeffer, J. M., Burke, J. P., Lodoen, F. V. (1970). Differentiation of norepinephrine storage compartments in peripheral adrenergic nerves. J. Pharmacol. exp. Ther. 174, 357–368 (1970).
Owman, Ch., Sjöberg, N. O.: Difference in rate of depletion and recovery of noradrenaline in “short” and “long” sympathetic nerves after reserpine treatment. Life Sci. 6, 2549–2556 (1967).
Ritzén, M.: Cytochemical identification and quantitation of biogenic monoamines. Thesis, Institute for Medical Cell Research and Genetics, Medical Nobel Institute, Karolinska Institute (1967).
Sheppard, H., Zimmerman, J.: Effect of guanethidine (SU-5864) on tissue catecholamines. Pharmacologist 1, 69 (1959).
Shore, P. A., Alpers, H. S., Busfield, D.: On the mechanism of norepinephrine depletion by reserpine, metaraminol and related compounds and antagonism by monoamine oxidase inhibition. In: Mechanism of release of biogenic amines, ed. Euler U. S. von, Rosell, S., and Uvnas, B., p. 319–328. Oxford: Pergamon Press 1965.
Sjöstrand, N. O., Swedin, G.: Effect of reserpine on the noradrenaline content of the vas deferens and the seminal vesicle compared with the submaxillary gland and the heart of the rat. Acta physiol. scand. 72, 370–377 (1968).
Stjärne, L.: Studies of catecholamine uptake, storage and release mechanisms. Acta physiol. scand. 62, Suppl. 228, 1–97 (1964).
Thoenen, H., Tranzer, J. P.: Chemical sympathectomy by selective destruction of adrenergic nerve endings with 6-hydroxydopamine. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 261, 271–288 (1968).
Tranzer, J. P., Thoenen, H.: An electron microscopic study of selective, acute degeneration of sympathetic nerve terminals after administration of 6-hydroxydopamine. Experientia (Basel) 24, 155–156 (1968).
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This work was supported by grants from the Australian Research Grants Committee and the National Health and Medical Research Council. We thank Prof. G. Burnstock for his support and for his critical reading of the manuscript. We are particularly grateful to Dr. H. Corrodi for a gift of FLA 63.
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Costa, M., Furness, J.B. Storage, uptake and synthesis of catecholamines in the intrinsic adrenergic neurones in the proximal colon of the guinea-pig. Z. Zellforsch. 120, 364–385 (1971). https://doi.org/10.1007/BF00324898
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DOI: https://doi.org/10.1007/BF00324898