Summary
Temperature sensitive suppression of nonsense mutations has been obtained, in E. coli, by inducing a mutation in the suppressor gene su-4, a structural gene for a tyrosine tRNA. In this mutant while no suppression is detectable at 42°C it is always present at lower temperatures. This temperature sensitivity is amenable to a reversible inactivation of the product of the su-4 gene.
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Gallucci, E., Pacchetti, G. & Zangrossi, S. Genetic studies on temperature sensitive nonsense suppression. Molec. Gen. Genetics 106, 362–370 (1970). https://doi.org/10.1007/BF00324053
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DOI: https://doi.org/10.1007/BF00324053