Summary
Two types of granules can be distinguished in the toad choroid plexus under the light microscope: pigment granules, mainly localized in the cells that line the free ends of the choroidal villi, and Gomori-positive granules, present in most epithelial cells.
The ultrastructural analysis of the choroid plexus reveals three types of granules: multivesicular bodies (MVB), multigranulous bodies (MGB) and dense bodies (DB), and intermediate stages between the last two bodies. The pigment granules seen under the light microscope probably correspond to the DB of the electron micrographs, and the Gomori-positive granules to the MGB. The probable role of these bodies is discussed and so is the significance of the glycogen present in the choroidal cells, their processes and endothelium.
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References
Baker, J. R.: Cytological technique. London: Methuen & Co. 1946.
Bargmann, W., u. E. Katritsis: Über die sog. Filamente und das Pigment im Plexus chorioideus des Menschen. Z. Zellforsch. 75, 366–370 (1966).
Bennett, H. S., and J. H. Luft: S-collidine as a basis for buffering fixatives. J. biophys. biochem. Cytol. 6, 113–114 (1959).
Biondi, G.: Zur Histopathologie des menschlichen Plexus chorioideus und des Ependyms. Arch. Psychiat. Nervenkr. 101, 666–728 (1934).
Bowsher, D.: Pathways of absorption of protein from the cerebrospinal fluid. An autoradiographic study in the cat. Anat. Rec. 128, 23–39 (1957).
Cameron, G.: Secretory activity of the choroid plexus in tissue culture. Anat. Rec. 117, 115–125 (1953).
Cavazos, L. F., W. D. Belt, M. N. Sheridan, and W. M. Feagans: The fine structure of the hamster seminal vesicle with special reference to pigment formation. Z. Zellforsch. 68, 179–193 (1964).
Flather, M. D.: A study of the haemosiderin content of the choroid plexus. Amer. J. Anat. 32, 125–153 (1923).
Gabe, M.: Quelques applications de la coloration par la fuchsine-paraldéhyde. Bull. Micr. appl. 3, 153–162 (1953).
Kappers, A. J.: On the presence of periodic acid Schiff positive substances in the paraphysis cerebri the choroid plexus and the neuroglia of Ambystoma mexicanum. Experientia (Basel) 12, 187–189 (1956).
—: Structural and functional changes in the telencephalic choroid plexus during human ontogenesis. In: The cerebrospinal fluid, p. 3. London: Churchill 1958.
Leduc, E. H., and G. B. Wislocki: The histochemical localization of acid and alkaline phosphatases, non-specific esterase and succinic dehydrogenase in the structures comprising the hematoencephalic barrier of the rat. J. comp. Neurol. 97, 241–280 (1952).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Lumsden, C. E.: Observations on the choroid plexus maintained as an organ in tissue culture. In: The cerebrospinal fluid, p. 97. London: Churchill 1958.
Millonig, G.: A modified procedure for lead staining of thin sections. J. biophys. biochem. Cytol. 11, 736–739 (1961).
Oksche, A.: Histologische Untersuchungen über die Bedeutung des Ependyms, der Glia und der Plexus choriodei für den Kohlenhydratstoffwechsel des ZNS. Z. Zellforsch. 48, 74–129 (1958).
Pappenheimer, J. R., S. R. Heisey, and E. F. Jordan: Active transport of diodrast and phenolsulfonphtalein from cerebrospinal fluid to blood. Amer. J. Physiol. 200, 1–10 (1961).
Pearse, A. G. E.: Histochemistry. Theoretical and applied. London: Churchill 1960.
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Rodriguez, E. M.: Neurosecretory system of the toad bufo arenarum Hensel and its changes during inanition. Gen. comp. Endocr. 4, 684–695 (1964).
—: Differences between median eminence and neural lobe in amphibians. Z. Zellforsch. 74, 308–316 (1966).
Tramezzani, J. H., C. Negreiros de Paiva, and A. Sesso: Oxytocic activity of the toads' brain. Acta endocr. 23, 175–184 (1956).
Volkmann, R. V.: Morphologie, Entstehung und Vorkommen des Abnutzungspigmentes im Epithel des menschlichen Plexus chorioideus. Z. Anat. Entwickl.-Gesch. 102, 211–231 (1933).
Wislocki, G. B., and E. W. Dempsey: The chemical cytology of the choroid plexus and bloodbrain barrier of the rhesus monkey (Macaca mulatta). J. comp. Neurol. 88, 319–345 (1948).
-, and A. J. Ladman: The fine structure of the mammalian choroid plexus. In: The cerebrospinal fluid, p. 55. London: 1958.
Wolff, F.: Funktionell-histologische Studien am Plexus chorioideus von Rana temporaria L. unter besonderer Berücksichtigung der Sekretionsfrage. Z. Zellforsch. 57, 63–105 (1962).
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This study was partially supported by the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina and the Rockefeller Foundation (School grant RF — 58028).
Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina. The author wishes to thank Prof. M. H. Burgos for his constant interest. His thanks are also due to Prof. H. Heller for providing certain facilities in his department and for his criticism.
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Rodriguez, E.M. Light and electron microscopy of granules in the toad choroid plexus. Zeitschrift für Zellforschung 82, 362–375 (1967). https://doi.org/10.1007/BF00323860
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DOI: https://doi.org/10.1007/BF00323860