Skip to main content
Log in

Studies on proteins of animal ribosomes

III. Immunochemical analyses of ribosomes from different tissues and species of animals

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Ribosomes from different tissues and species of animals were tested by several different immunochemical methods. With antisera produced in rabbits by injection of intact ribosomes significant species differences in the antigenic properties of the ribosomes could be demonstrated whereas no tissue conditioned properties in the antigenic determinants were found.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alberghina, F. A. M., Suskind, S. R.: Ribosomes and ribosomal protein from Neurospora crassa. I. Physical, chemical, and immunochemical properties. J. Bact. 94, 630 (1967).

    Google Scholar 

  • Bielka, H., Noll, F.: Antigenic properties of ribosomes from animal tissues. Acta biol. med. germ. 21, K1 (1968).

    Google Scholar 

  • Bielka, H., Welfle, H.: Characterization of ribosomal proteins from different tissues and species of animals by electrophoresis on polyacrylamide gel. Molec. Gen. Genetics 102, 128 (1968).

    Google Scholar 

  • —, Böttger, M., Förster, W.: Strukturveränderungen und Dissoziation von Leberribosomen in Abhängigkeit von der Mg++-Konzentration. Europ. J. Biochem. 5, 183 (1968).

    Google Scholar 

  • Frey, J. R., Decken, A. von der, Hultin, T.: Antigenic properties of acid-soluble ribosomal proteins of Artemia salina. Exp. Cell Res. 53, 615 (1968).

    Google Scholar 

  • Friedman, D. J., Olenick, J. G., Hahn, F. E.: A 50s ribosomal determinant: Immunological studies correlating function and structure. J. molec. Biol. 32, 579 (1968).

    Google Scholar 

  • — Wolfe, A. D., Olenick, J. G.: Immunological identification of a ribosomal protein on a subribosomal particle. J. molec. Biol. 41, 305 (1969).

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265 (1951).

    Google Scholar 

  • Panijel, J., Delannay, M. Ch.: An immunological approach to the structure of the ribosomal particles. Biochem. biophys. Res. Commun. 29, 158 (1967).

    Google Scholar 

  • Quash, G., Dandeu, J. P., Barbu, E., Panijel, J.: Recherches préliminaires sur les antigens des ribosomes. Ann. Inst. Pasteur 103, 3 (1962).

    Google Scholar 

  • Rothschild, H., Itikawa, H., Suskind, S. R.: Ribosomes and ribosomal proteins from Neurospora crassa. II. Ribosomal proteins in different wild-type strains and during various stages of development. J. Bact. 94, 1800 (1967).

    Google Scholar 

  • Schur, P. H., Moroz, L. A., Kunkel, H. G.: Precipitating antibodies to ribosomes in the serum of patients with systemic Lupus erythematosus. Immunochemistry 4, 447 (1967).

    Google Scholar 

  • Stavitsky, A. B.: Haemagglutination and haemagglutination inhibition reactions with tannic-acid and BDB-protein conjugated erythrocytes. In: J. F. Ackroyd, Immunological methods, p. 363. Oxford: Blackwell Sci. Publ. 1964.

    Google Scholar 

  • Wasserman, E., Levine, L.: Quantitative micro-complement-fixation and its use in the study of antigenic structure by specific antigen-antibody-inhibition. J. Immunol. 87, 290 (1961).

    Google Scholar 

  • Welfle, H., Bielka, H., Böttger, M.: Studies on proteins of animals ribosomes. II. Separation of ribosomal proteins from rat liver by preparative polyacrylamide gel electrophoresis and some properties of the protein fractions. Molec. Gen. Genetics 104, 165 (1969).

    Google Scholar 

  • Westermann, P., Bielka, H., Böttger, M.: Studies on proteins of animal ribosomes. I. Separation of ribosomal proteins from rat liver by chromatography on CM-cellulose and properties of some protein components. Molec. Gen. Genetics 104, 157 (1969).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by H. G. Wittmann

Abbreviations. RRL: ribosomes of rat liver; RBL: ribosomes of bovine liver; RBK: ribosomes of bovine kidney; anti-RRL: antiserum against RRL; anti-RBL: antiserum against RBL; anti-RBK: antiserum against RBK.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Noll, F., Bielka, H. Studies on proteins of animal ribosomes. Molec. Gen. Genet. 106, 106–113 (1970). https://doi.org/10.1007/BF00323829

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00323829

Keywords

Navigation