Summary
Dry seeds of spring barley variety MOS-121 were treated with ethyleneimine and after the treatment were stored in the desiccator over granulated KOH from 6 to 46 days. During this period statistically significant changes in the general level of chromosome rearrangements, number of chlorophyll mutations, death-rate and fertility of M 1-plants were observed. The spectrum of chlorophyll mutations also changed. The maximum genetic effect resulting from storage was noted by the 18th day, and this was followed by the decrease of injuries reached zero by the 46th day.
The mode of changes in dynamics of the formation of chromosome aberrations, chlorophyll mutations and recessive lethal mutations proves the similar nature of the initial mechanisms of their potential changes.
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Bender, K., Gaul: Variierung der AMS-Wirkung bei Gerste durch Anwendung verschiedener Behandlungs- und Nachwaschtemperaturen. Radiat. Bot. 7, 289–301 (1967).
Dubinin, N. P.: On some main questions of modern mutation theory. Genetics 7, 3 (1966).
— Dubinina, L. G.: The problem of conduction of potential changes in chromosomes in the process of storage of Crepis capillaris dry seeds. Genetics 4, 9, 5–23 (1968).
Dubinina, L. G., Dubinin, N. P.: The proofs of conduction of lesions in chromosomes by alkylating agents in the presinthetical phase of the cell cycle. Report USSR Acad. Sci. 175, 1, 213 (1967).
— New in the action of alkylating agents on chromosome mutations. Genetics 4, 2, 5 (1968).
Ehrenberg, L., Gustafsson, A., Lundquist, U.: The mutagenic effects of ionizing radiations and reactive ethylene derivatives in barley. Hereditas (Lund) 45 (1959).
—, Lundquist, U., Osterman, S., Sparrman, B.: On the mutagenic action of alkane-sulfonic esters in barley. Hereditas (Lund) 56, 277–305 (1966).
Froese-Gertzen, E., Konzak, C., Nilan, R., Heiner, R.: The effects of ethylmethanesulfonate on the growth response, chromosome structure and mutation rate in barley. Radiat. Bot. 4, 61–69 (1964).
Gichner, T., Ehrenberg, L.: The influence of post-treatment storage on the frequency of EMS-inducted chromosomal aberration in barley. Biol. Plant. Acad. Sci. (Bohemoslov) 8, 256–259 (1966).
Gustafsson, A.: The mutation system of the chlorophyll apparatus. Lunds Univ. Årsskr., N.F. 2, 36, 11, 1–40 (1940).
—, Lundquist, U., Ekberg, J.: Yield reactions and rates origin of chromosome rearrangements and gene mutations in barley. Hereditas (Lund) 56, 200 (1966).
Konzak, C. F., Nilan, R. A., Froese-Gertzen, E. E., Foster, R. I.: Factors affecting the biological action of mutagenes. In: Induction of mutations and the mutation process, p. 123. Prague 1965.
Mikaelsen, K.: Studies on mutagenic effects of ionizing radiation and chemical mutagens IAEA Techn. Rep. Ser. (Vienna) 77, 50–57 (1967).
—, Ahnström, G., Li, W. C.: Genetic effects of alkylating agents in barley. Influence of post-storage, metabolic state and pH of mutagen solution. Hereditas (Lund) 59, 353–374 (1968).
Müller, A. J.: Reparation chemisch induzierter prämutativer Läsionen durch Rücktrocknung der behandelten Samen. Biol. Lbl. 84, 759–762 (1965).
—: The influence of post-treatment-storage of seeds on mutation induction with alkilmethane-sulfonates, nitrosamides and X-rays. Arab. Inf. Serv. 4, 43 (1967).
Natarajan, A. T., Shivasankar, G.: Studies on modification of mutation response of barley seeds to ethyl methanesulfonate. Z. Vererbungsl. (lb) 96, 1, 13–21 (1965).
Nilan, R. A., Konzak, C. F., Heiner, R. E., Froese-Gertzen, E.: Chemical mutagenesis in barley. Barley Genetics I. Proceedings of the First Internat. Barley Genetics Symposium, Wageningen, 35–54 (1963).
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Garina, K.P., Romanova, N.I. The influence of post-treatment storage on the frequency of ethyleneimine-induced chromosomal aberrations and gene mutations in barley. Molec. Gen. Genet. 106, 93–105 (1970). https://doi.org/10.1007/BF00323828
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DOI: https://doi.org/10.1007/BF00323828