Skip to main content
SpringerLink
Log in

Sensitive method for the measurement of citrate lyase activity by differential pulse polarography

  • Original Papers
  • Biochemical/Clinical Analysis
  • Published:
Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Summary

A method is described for the determination of citrate lyase (E.C. 4.1.3.6) activity using differential pulse polarography. It is based on the measurement of the reduction wave of the reaction product pyruvate in the presence of an excess of citrate as the substrate. The method is very sensitive and rapid. Additionally, kinetic parameters and the Michaelis constant (Km) can be obtained.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bergmeyer HU (1983) Methods of enzymatic analysis, 3rd edn, vol I. VCH, Weinheim

    Google Scholar 

  2. Takahashi H, Takahara S (1968) J Biochem 64:7

    Google Scholar 

  3. Majchrowicz E (1975) Biochemical pharmacology of ethanol, vol. 56. Plenum, New York

    Google Scholar 

  4. Taylor R (1985) Anal Chim Acta 172:241

    Google Scholar 

  5. Hasebe K, Hikima S, Yoshida H (1990) Fresenius J Anal Chem 338:813

    Google Scholar 

  6. Hasebe K, Hikima S, Yoshida H (1991) Fresenius J Anal Chem 339:261

    Google Scholar 

  7. Sulaiman ST, Saleen MMN (1985) Analyst (London) 110:1193

    Google Scholar 

  8. Hasebe K, Hikima S, Kakizaki T, Yoshida H (1989) Fresenius Z Anal Chem 333:19

    Google Scholar 

  9. Hasebe K, Hikima S, Yoshida H (1990) Fresenius J Anal Chem 336:232

    Google Scholar 

  10. Wheat RW, Ajl SJ (1955) J Biol Chem 217:897

    Google Scholar 

  11. Daron HH, Gunsalus IC (1962) Methods Enzymol 5:622

    Google Scholar 

  12. Wheat RW, Ajl SJ (1955) J Biol Chem 217:909

    Google Scholar 

  13. Krampitz LO, Werkman CH (1941) Biochem J 35:595

    Google Scholar 

  14. Bauer E (1971) A statistical manual for chemists, 2nd ed. Academic Press, New York

    Google Scholar 

Download references

Author information

Author notes

  1. Kiyoshi Hasebe

    Present address: Graduate School of Environmental Earth Science, Hokkaido University, 060, Sapporo, Japan

Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Hokkaido University, 060, Sapporo, Japan

    Kiyoshi Hasebe, Satoshi Hikima & Yukimi Yamamoto

  2. Hokkaido University of Education at Iwamizawa, 068, Iwamizawa, Japan

    Teiji Kakizaki

Authors
  1. Kiyoshi Hasebe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satoshi Hikima
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yukimi Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Teiji Kakizaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hasebe, K., Hikima, S., Yamamoto, Y. et al. Sensitive method for the measurement of citrate lyase activity by differential pulse polarography. Fresenius J Anal Chem 347, 368–370 (1993). https://doi.org/10.1007/BF00323823

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00323823

Keywords

Search

Navigation