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Development of an automated flow injection chemiluminescence immunoassay for human immunoglobulin G

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  • Clinical Analysis
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Abstract

The development of a competitive solid phase flow-injection immunoassay with on-column chemiluminescence detection is described. The immunoreactor column consists of a transparent teflon tubing packed with immobilized antibodies. It is placed in front of the photomultiplier tube. The entire assay takes place in this immunoreactor cell. The assay is performed by injection of a mixture of sample HIgG and acridinium labeled HIgG followed by hydrogen peroxide to initiate the chemiluminescence reaction. One assay cycle including regeneration takes 7 min. The system is fully automated and controlled by computer to ensure exact timing resulting in a precision of 1 to 3%, dependent on concentration. The detection limit was 7 fmol. The method was applied to serum samples without any sample clean-up. The average recovery from spiked serum samples was 103%.

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Authors and Affiliations

  1. Institute of Pharmaceutical Chemistry, Karl Franzens Universität of Graz, Universitätsplatz 1, A-8010, Graz, Austria

    Andrea Hacker, Martin Hinterleitner & Gerald Gübitz

  2. Sanofi Diagnostica Pasteur Inc., 1000 Lake Hazeltine Drive, 55318-1084, Chaska, MN, USA

    Curt Shellum

Authors
  1. Andrea Hacker
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  2. Martin Hinterleitner
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  3. Curt Shellum
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  4. Gerald Gübitz
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Additional information

Dedicated to Professor Dr. Dr. h.c. mult. J.F.K. Huber on the occasion of his 70 birthday

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Hacker, A., Hinterleitner, M., Shellum, C. et al. Development of an automated flow injection chemiluminescence immunoassay for human immunoglobulin G. Fresenius J Anal Chem 352, 793–796 (1995). https://doi.org/10.1007/BF00323066

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  • DOI: https://doi.org/10.1007/BF00323066

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