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Fluorometric determination of hydrogen peroxide with peroxidase and 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid

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  • Clinical And Biological Analysis
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Summary

The possibility of the fluorometric determination of hydrogen peroxide using various tetrahydro-β-carbolines as hydrogen-donating substrates for horseradish peroxidase was examined. A simple and sensitive method has been developed by using 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid as a substrate. The fluorescence intensity was proportional to the hydrogen peroxide concentration in the range of 0.05–1 μmol/l (30–600 pmol/tube) in the sample solution with a relative standard deviation of 3.8% (0.1 μmol/l).

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Authors and Affiliations

  1. Faculty of Pharmaceutical Seiences, Science University of Tokyo, 12 Ichigaya-Funagawara-machi, Shinjuku-ku, 162, Tokyo, Japan

    Takafumi Ohta, Yuki Yamauchi & Shoji Takitani

Authors
  1. Takafumi Ohta
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  2. Yuki Yamauchi
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  3. Shoji Takitani
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Ohta, T., Yamauchi, Y. & Takitani, S. Fluorometric determination of hydrogen peroxide with peroxidase and 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid. Fresenius J Anal Chem 343, 550–552 (1992). https://doi.org/10.1007/BF00322166

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  • DOI: https://doi.org/10.1007/BF00322166

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