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The integrated multivariate approach for the development of dry reagent strips for quantitative clinical analysis

  • Original Papers
  • Clinical And Biological Analysis
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Summary

The paper describes the development and the optimization of a visually and metrically evaluable dry reagent strip for quantitative clinical analysis of blood glucose based upon an enzymatic oxidation connected with a following colour coupling reaction. In contrast to classical experimental strategies, multivariate statistical methods were used to design a complex biochemical system with optimal properties. Former experiments were evaluated by means of factor analysis and the relations between the 12 biochemical variables and the 6 strip properties were studied. The mathematical discrimination between biochemically more and less relevant variables allowed a reduction of the number of experiments by a statistically reasonable design of additional selected experiments. By application of a pattern recognition technique and multivariate linear regression the data space and the variables' space were analysed. A following multivariate prediction using the partial least-squares technique yielded a glucose test strip with high reproducibility and long-term thermal stability.

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Authors and Affiliations

  1. Institut für Biochemie, Medizinische Akademie “Carl Gustav Carus” Dresden, Karl-Marx-Strasse 3, O-8080, Dresden, Federal Republic of Germany

    M. Ehrlich

  2. Laboratorium voor Analytische Chemie, Katholieke Universiteit Nijmegen, Toermooiveld 1, NL-6525 ED, Nijmegen, The Netherlands

    D. Wienke & G. Kateman

  3. Institut für Anorganische und Analytische Chemie, Friedrich-Schiller-Universität Jena, Fraunhofer-Strasse 6, O-6900, Jena, Federal Republic of Germany

    K. Danzer

Authors
  1. M. Ehrlich
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  2. D. Wienke
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  3. K. Danzer
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  4. G. Kateman
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Ehrlich, M., Wienke, D., Danzer, K. et al. The integrated multivariate approach for the development of dry reagent strips for quantitative clinical analysis. Fresenius J Anal Chem 343, 532–540 (1992). https://doi.org/10.1007/BF00322164

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  • DOI: https://doi.org/10.1007/BF00322164

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