Abstract
A rapid field screening test for 2,4,6-trinitrotoluene (TNT) in water and soil is described. The immunofiltration assay is based on a simplified enzymelinked immunosorbent assay (ELISA) performed in a pre-packed portable device. Performance of the test was assayed in spiked water, methanolic soil extracts (dilution 1: 10) and natural water samples. A quantitative colour response to concentrations of TNT in the range of 1 to 30 μg/l in water and 50 to 1000 μg/kg in soil is demonstrated. The relative average standard deviations are 11.9% and 14.1%, respectively. High correlations between immunofiltration, ELISA and gas chromatography have been demonstrated. The cross reactivities of the antibody are comparable in both techniques.
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Dedicated to Professor Dr. Dieter Klockow on the occasion of his 60th birthday
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Keuchel, C., Niessner, R. Rapid field screening test for determination of 2,4,6-trinitrotoluene in water and soil with immunofiltration. Fresenius J Anal Chem 350, 538–543 (1994). https://doi.org/10.1007/BF00321802
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DOI: https://doi.org/10.1007/BF00321802