Simultaneous spectrophotometric determination of fat-soluble vitamins in multivitamin pharmaceutical preparations


A spectrophotometric method is proposed for the simultaneous determination of vitamins A, D and E in multivitamin pharmaceutical preparations. This is based on multiple linear regression. Most vitamins are directly extracted from the preparations into n-hexane. Microen-capsulated vitamin A preparations require pretreatment of de-encapsulation before the vitamin is extracted. The wavelength range to be used for each preparation and the optimum spectral mode (absorbance or first-derivative) has been chosen in order to assure correct quantitation and avoid interferences from other absorbing species also extracted by n-hexane. The results obtained were validated by simultaneous HPLC analyses for accuracy and precision.

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Correspondence to M. Blanco.

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Blanco, M., Coello, J., Iturriaga, H. et al. Simultaneous spectrophotometric determination of fat-soluble vitamins in multivitamin pharmaceutical preparations. Fresenius J Anal Chem 351, 315–319 (1995).

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  • Physical Chemistry
  • Analytical Chemistry
  • HPLC
  • Linear Regression
  • Inorganic Chemistry