Simultaneous spectrophotometric determination of fat-soluble vitamins in multivitamin pharmaceutical preparations

Abstract

A spectrophotometric method is proposed for the simultaneous determination of vitamins A, D and E in multivitamin pharmaceutical preparations. This is based on multiple linear regression. Most vitamins are directly extracted from the preparations into n-hexane. Microen-capsulated vitamin A preparations require pretreatment of de-encapsulation before the vitamin is extracted. The wavelength range to be used for each preparation and the optimum spectral mode (absorbance or first-derivative) has been chosen in order to assure correct quantitation and avoid interferences from other absorbing species also extracted by n-hexane. The results obtained were validated by simultaneous HPLC analyses for accuracy and precision.

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References

  1. 1.

    United States Pharmacopeia, XXIIth Revision (1990) US Pharmacopeial Convention, Rockville, MD

  2. 2.

    Barnett SA, FricK LW (1979) Anal Chem 51:641–645

    Google Scholar 

  3. 3.

    Eriksson M, Eriksson T, Sörensen B (1978) Acta Pharm Suec 15:274–281

    Google Scholar 

  4. 4.

    Wahbi AM, Abounassif MA, Alkahtoni HMG (1989) J Pharm Biomed Anal 7:39–43

    Google Scholar 

  5. 5.

    Barary MH, Abdel Hamid ME, Hassan EM, El-Sayed MA (1985) Anal Lett 18:1835–1846

    Google Scholar 

  6. 6.

    Bukovits JG, Lezerovich A (1987) J Am Oil Chem Soc 64:517–520

    Google Scholar 

  7. 7.

    A. Fattah El Walily, El-Anwar F, Zamel S (1991) Anal Chim Acta 248:583–587

    Google Scholar 

  8. 8.

    Otto M, Wegscheider W (1985) Anal Chem 57:63–69

    Google Scholar 

  9. 9.

    Mattoon TR, McSwiggen P, George SA (1985) Printed Circuit Plating Bath Process Control (part I, II. III), Hewlett-Packard

  10. 10.

    Sternberg JC, Stillo HS, Schwendeman RH (1960) Anal Chem 32:84–90

    Google Scholar 

  11. 11.

    Fisher BV, Jones R (1987) J Pharm Biomed Anal 5:455–567

    Google Scholar 

  12. 12.

    Sala G, Maspoch S, Iturriaga H, Blanco M, Cerdà V (1988) J Pharm Biomed Anal 6:765–772

    Google Scholar 

  13. 13.

    Rossi DT, Pardue HL (1985) Anal Chim Acta 175:153–161

    Google Scholar 

  14. 14.

    Tahboub YR, Pardue HL (1985) Anal Chem 57:38–41

    Google Scholar 

Download references

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Correspondence to M. Blanco.

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Blanco, M., Coello, J., Iturriaga, H. et al. Simultaneous spectrophotometric determination of fat-soluble vitamins in multivitamin pharmaceutical preparations. Fresenius J Anal Chem 351, 315–319 (1995). https://doi.org/10.1007/BF00321656

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Keywords

  • Physical Chemistry
  • Analytical Chemistry
  • HPLC
  • Linear Regression
  • Inorganic Chemistry