Abstract
The interrelationships of a number of Crustacea were measured by nucleic acid hybridization techniques, with special emphas is on the question of whether GC-rich satellite DNA contains nucleotide sequences homologous to sequences found in other Crustacea with and without similar satellite DNAs. Repetitious sequences from both main-band DNA and GC-rich satellite DNA from the land crab, Gecarcinus lateralis, were hybridized to the total DNAs of crustaceans ranging from the brine shrimp (Subclass: Branchiopoda) to the North American lobster (Homarus americanus, Subclass: Malacostraca; Suborder: Repantia; Section: Macrura) and the true crabs (Subclass: Malacostraca; Suborder: Reptantia; Section: Brachyura). Approximately half of the Gecarcinus repetitious main-band DNA sequences were found to be represented in the DNA of the other true crabs, while a lesser but still significant amount of homology (5 to 10%) to the GC-rich satellite DNA was observed. We also observed a significant amount of homology of the Gecarcinus GC-rich satellite to other crustacean DNAs, even at the level of a different taxonomic Section. This is the first observation of hybrid formation between a purified satellite and DNAs from other organisms under stringent hybridization conditions.
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Research sponsored by the U.S. Atomic Energy Commission under contact with the Union Carbide Corporation.
Research performed while an Oak Ridge Graduate Fellow under appointment from the Oak Ridge Associated Universities in partial fulfillment of the Ph. D. degree from the University of Tennessee, Knoxville, Tennessee.
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Graham, D.E., Skinner, D.M. Homologies of repetitive DNA sequences among Crustacea . Chromosoma 40, 135–152 (1972). https://doi.org/10.1007/BF00321459
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DOI: https://doi.org/10.1007/BF00321459