Summary
Cysteine auxotrophy and absence of cystathionase (CSE) has been associated with certain human and rodent leukemic cell lines. To determine whether this state was a marker of malignant transformation or of cellular differentiation, CSE content was measured in 16 well characterized human leukemia-lymphoma cell lines. Enzyme was easily detected in several lines but its level did not correlate with a proposed scheme of differentiation based on cell-surface markers. However, the apparent absence of enzyme in human bone marrow CFU-C determined by growth experiments suggests reduced levels of CSE may be a marker of cytoplasmic immaturity.
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References
Boyum A (1964) Separation of white blood cells. Nature 204: 793
Burgess AW, Wilson CMA, Metcalf D (1977) Stimulation by human placental conditioned medium of hematopoiesis colony formation by human marrow cells. Blood 49: 573
Gaull G, Sturman JA, Raiha N (1972) Development of mammalian sulfur metabolism: Absence of cystathionase in human fetal tissues. Pediatr Res 6: 538
Glode LM, Epstein A, Smith CG (1981) Reduced gamma cystathionase protein content in human malignant leukemia cell lines as measured by immunoassay with monoclonal antibody. Cancer Res 41: 2249
Glode LM, Greene HL, Bikel I (1979) Cystathionase in normal and leukemic cells. Cancer Treat Rep 63: 1081
Glode LM, Kriegler MP, Livingston DM (1979) Cysteine auxotrophy of human leukemic lymphoblasts is associated with decreased amounts of intracellular cystathionase protein. Biochemistry 20: 1306
Greengard O, Herzfeld A (1977) The undifferentiated enzymic composition of human fetal lung and pulmonary tumor. Cancer Res 3: 884
Iglehart JD, York R, Modest AP, Lazarus H, Livingston DM (1977) Cysteine requirements of continuous human lymphoid cell lines of normal and leukemic origins. J Biol Chem 252: 7184
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680
Livingston DM, Ferguson C, Gollogly K, Lazarus H (1976) Accumulation of cysteine auxotrophic thymocytes accompanying type C viral leukemogenesis in the mouse. Cell 7: 41
Lowry OH, Rosebrough MJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193: 265
Matsuo Y, Greenberg DM (1958) A crystalline enzyme that cleaves homoserine and cystathionine. I. Isolation procedure and some physiochemical properties. J Biol Chem 230: 545
Minowada J, Toshiba H, Sagawa K, Kubonishi I, Lok MS, Tatsumi E, Han T, Srivastava BIS, Ohnuma T (1981) Marker profiles of human leukemia and lymphoma cell lines. J Cancer Res Clin Oncol 101: 91
Ohnuma T, Waliguada J, Holland JF (1971) Amino acid requirements in vitro of human leukemic cells. Cancer Res 31: 1640
Pike BL, Robinson WA (1970) Human bone marrow colony growth in agar-gel. J Cell Physiol 76: 77
Rose WL, Wixom RL (1955) The amino acid requirements of man. XIII. The sparing effect of cystine on the methionine requirement. J Biol Chem 216: 763
Uren J, Lazarus H (1979) L-cyst[e]ine requirements of malignant cells and progress toward depletion therapy. Cancer Treat Rep 63: 1073
Uren J, Ragin R, Chaykovsky M (1978) Modulation of cysteine metabolism in mice: Effects of propargylglycine and L-cyst[e]ine-regrading enzymes. Biochem Pharmacol 27: 2807
Weisberger AS, Levine B (1954) Incorporation of radioactive L-cystine by normal and leukemic leukocytes in vivo. Blood 9: 1082
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Supported by a grant from the National Institutes of Health (CA29331-01 1 RO1). Dr. Glode is a Junior Faculty Clinical Fellow of the American Cancer Society
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Link, D., Drebing, C. & Glode, L.M. Cystathionase: A potential cytoplasmic marker of hematopoietic differentiation. Blut 47, 31–39 (1983). https://doi.org/10.1007/BF00321048
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DOI: https://doi.org/10.1007/BF00321048