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Immunohistochemical detection of pregnancy and placental proteins in human and cynomolgus monkey (macaca fascicularis) peripheral blood cells

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Summary

With use of an enzyme-bridge immunoperoxidase method, the localization of pregnancy-specific β1-glycoprotein (SP1), pregnancy-associated proteins (SP2, SP3) and placental tissue proteins (FSF, PP1, PP2 and PP4 to PP12) was investigated in human and cynomolgus peripheral blood cells.

Blood cells of cynomolgus monkeys revealed the same staining results as in man: SP1, SP2, SP3, PP2, PP4, PP6, PP7, PP8, PP10 and PP12 were found to be positive in the polymorphonuclear neutrophils. Monocyte macrophages stained positively only for PP2 and PP10. Lymphocytes and erythrocytes did not stain positively for any of the proteins examined.

A much stronger positive staining for SP2 and SP3 was found in the polymorphonuclear neutrophils from a human female taking contraceptive pills and pregnant cynomolgus monkeys. Otherwise there was no difference in staining results irrespective of whether the specimens were from males, or pregnant or non-pregnant females.

The results reported here indicate that the so-called placenta-“specific” proteins SP1, PP10 and PP12 are apparently also synthesized by normal blood cells and appear to be not specific to the placenta. Only PP5 and PP11 seem to be highly specific to the trophoblast.

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Authors and Affiliations

  1. Research Laboratories, Behringwerke AG, Postfach 1140, D-3550, Marburg (Lahn), Federal Republic of Germany

    N. Inaba, T. Renk, W. Ax, E. Weinmann, S. Schottler & H. Bohn

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  1. N. Inaba
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  2. T. Renk
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  3. W. Ax
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  4. E. Weinmann
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  6. H. Bohn
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Inaba, N., Renk, T., Ax, W. et al. Immunohistochemical detection of pregnancy and placental proteins in human and cynomolgus monkey (macaca fascicularis) peripheral blood cells. Blut 43, 315–323 (1981). https://doi.org/10.1007/BF00320955

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  • DOI: https://doi.org/10.1007/BF00320955

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