Summary
DBA/2 mice were infected with the polycythemia inducing Friend Virus (F-MuLV-P) and treated with different doses of Actinomycin D (Act D) when the whole erythroid cell system, as measured by the CFU-E technique with and without addition of erythropoietin (Ep), had been transformed into Ep-independence. During and after this therapy the different stem cell pools CFU-S, CFU-C, BFU-E and CFU-E (with and without Ep) were studied and their sensitivity to Act D in bone marrow and spleen was compared to that of normal mice, recently published by other authors. There seemed to be no difference in the Act D sensitivity between normal erythropoiesis and Ep-independent erythropoiesis caused by F-MuLV-P. Furthermore a cell called ICPC (infectious centers producing cell) was studied. This cell system, detected by spleen colony formation due to high local virus production in an unirradiated host, proved to be Act D sensitive in the spleen but not in the marrow. When the erythroid cell system regenerated after Act D chemotherapy, all erythroid colony growth was Ep-independent. This means that Act D did not induce normal erythropoiesis as seen with hydroxyurea treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Axelrad AA, McLeod DL, Shreeve MM, Heath DS (1974) Properties of cells that produce erythrocytic colonies in vitro. In: Robinson, WA (ed) Proc Second International Workshop on Hematopoiesis in Culture, US Government Printing Office, Washington, p 226
Iscove NN, Sieber F (1975) Erythroid progenitors in mouse bone marrow detected by macroscopic colony formation in culture. Exp Hematol 3: 32
Iscove NN, Sieber F, Winterhalter KH (1974) Erythroid colony formation in cultures of mouse and human marrow: Analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A. J Cell Physiol 83: 309
Iscove NN, Melchers F (1978) Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med 147: 923
Krantz SB, Gallien-Lartigue O, Goldwasser E (1965) On the mechanism of erythropoietin-induced differentiation. II. The effect on RNA synthesis. Biochim Biophys Acta 103: 325
Opitz U, Seidel HJ, Bertoncello I (1978) Erythroid stem cells in Friend-virus infected mice. J Cell Physiol 96: 95
Opitz U, Seidel HJ (1978) Studies on the target cell for the Friend virus (FV-P strain) using the CFU-E technique. Blut 37: 183
Peschle C, Migliaccio G, Lettieri F, Migliaccio AR, Ceccarelli R, Barba P, Titti F, Rossi GB (1980) Kinetics of erythroid precursors in mice infected with the anemic or the polycythemic strain of Friend leukemia virus. Proc Natl Acad Sci USA 77: 2054
Reissman KR, Ito K (1966) Selective eradication of erythropoiesis by actinomycin D as the result of interference with hormonally controlled effector pathway of cell differentiation. Blood 28: 201
Rich I, Heit W, Kubanek B (1977) The long and short term effect of Actinomycin D on erythropoiesis. Exp Hematol [Suppl 2] 5: 78
Rich I, Heit W, Kubanek B (1980) The effect of actinomycin D on hemopoiesis. I. Short-term effects. Blut 41: 29–40
Seidel HJ, Opitz U (1979) Effect of Actinomycin D and Busulphan on stem cells in normal and Friend virus infected mice. Blut 38: 349–360
Seidel HJ, Opitz U (1979) Hemopoietic stem cells in Friend virus infected mice under chemotherapy. Remission and relapse of Ep-independent erythropoiesis induced by hydroxyurea. J Natl Cancer Inst 62: 531
Steeves RA, Bubbers JE, Plata F, Lilly F (1978) Origin of spleen colonies generated by Friend virus-infected cells. Cancer Res 38: 2729
Steeves RA, Mirand EA, Price FW (1968) Effect of hydroxyurea and actinomycin D on several parameters of infection by Friend spleen focus-forming virus. Cancer Chemother Rep 52: 557
Steinheider G, Bertoncello I, Seidel HJ (1980) Characterization of early leukemic cells in spleen and bone marrow of Friend virus infected mice. Exp Hematol 8: 779
Tambourin PE, Wendling F, Jasmin C, Smadja-Joffe F (1979) The physiopathology of Friend leukemia. Leukemia Res 3: 117
Thomson S, Axelrad AA (1968) A quantitative spleen colony assay method for tumor cells induced by Friend leukemia virus infection in mice. Cancer Res 28: 2105
Wendling F, Tambourin PE, Jullien P (1972) Hematopoietic CFU in mice infected by the polycythemia-inducing Friend virus. I. Number of CFU, and differentiation pattern in the spleen colonies. Int J Cancer 9: 554
Wendling F, Tambourin PE (1978) Oncogenicity of Friend virus infected cells. Determination of origin of spleen colonies by the H-2 antigens as genetic markers. Int J Cancer 22: 479
Zuckerman KS, Sullivan R, Quesenberry P (1978) Effects of Actinomycin D in vivo on murine erythroid stem cells. Blood 51: 957
Author information
Authors and Affiliations
Additional information
Supported by Deutsche Forschungsgemeinschaft, (SFB 112) and Stiftung Volkswagenwerk
With assistance of E. Barthel, M. Grünefeld, and K. Steinhoff
Rights and permissions
About this article
Cite this article
Seidel, H.J., Kreja, L., Opitz, U. et al. Normal and friend virus specific cell compartments in friend leukemia characterized by their sensitivity to actinomycin D in vivo. Blut 43, 287–296 (1981). https://doi.org/10.1007/BF00320952
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00320952