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Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (TÜ 67)

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Summary

Anti-TÜ 67 is a murine monoclonal antibody that recognizes the transferrin receptor. With respect to hematopoietic cells TÜ 67 is expressed by human multipotent colony-forming cells (CFU-Mix), erythroid progenitor cells (BFU-E and CFU-E) and a fraction of granulocyte/monocyte colony forming cells, but is not expressed by mature hematopoietic cells including erythrocytes, platelets, lymphocytes, and peripheral blood myeloid cells. The TÜ 67-positive fraction of normal bone marrow, separated by fluorescence-activated cell sorting (FACS) or immune rosettes, contained 87% of the erythroid progenitor cells. Erythroid progenitor cells were enriched up to 50-fold by using a combination of monoclonal antibodies to deplete mature hematopoietic cells, followed by positive selection of BFU-E and CFU-E by TÜ 67 antibody.

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Authors and Affiliations

  1. Abteilung für Hämatologie, I. Medizinische Klinik, Johannes Gutenberg-Universität, D-6500, Mainz, Federal Republic of Germany

    F. Herrmann, J. D. Griffin, K. D. Sabbath, W. Oster, P. Wernet & R. Mertelsmann

  2. Division of Tumor Immunology, Dana-Farber Cancer Institute, 02115, Boston, MA, USA

    F. Herrmann, J. D. Griffin, W. Oster & R. Mertelsmann

  3. Roger Williams Cancer Center, Brown University, 02908, Providence, RI, USA

    F. Herrmann, K. D. Sabbath, W. Oster & R. Mertelsmann

  4. Medizinische Klinik, Abteilung Innere Medizin II, Immunhämatologisches Labor, Eberhard-Karls-Universität, D-7400, Tübingen, Federal Republic of Germany

    F. Herrmann, W. Oster, P. Wernet & R. Mertelsmann

Authors
  1. F. Herrmann
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  2. J. D. Griffin
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  3. K. D. Sabbath
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  4. W. Oster
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  5. P. Wernet
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  6. R. Mertelsmann
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Supported by the Deutsche Forschungsgemeinschaft (He 1380-2/1)

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Herrmann, F., Griffin, J.D., Sabbath, K.D. et al. Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (TÜ 67). Blut 56, 179–183 (1988). https://doi.org/10.1007/BF00320749

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  • DOI: https://doi.org/10.1007/BF00320749

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