Summary
The effect of Gey's balanced salt solution deficient in NaCl on cells in tissue culture led to a study of various solutions employed as vehicles for plasma expanders.
The behavior of epithelium from the adult human mucosa, tonsils from children, and mouse kidney papilla was recorded in a perfusion chamber with phase contrast, time-lapse cinematography. After perfusion for one to two hours with fluid nutrient medium, test solutions were brought in contact with the cells for one hour. The fluid system was then generally replaced with Gey's balanced salt solution and, or, the proteinaceous nutrient medium.
So-called normal saline produced marked injury both when employed alone or in combination with 6% Dextran. Damage resulting from 5% dextrose either alone or in combination with 6% Dextran was characterized by vacuole formation. Dextran at 6% in Gey's balanced salt solution or in Ringer's solution appeared to produce little injury to cells under the conditions of these experiments.
Work in progress is designed to examine the lifesaving value of the various solutions in the treatment of partially exsanguinated dogs.
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This investigation was supported by the Medical Research and Development Board, Office of the Surgeon General, Department of the Army, under Contract No. DA-49-007-MD-32.
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Pomerat, C.M., Overman, R.R. Electrolytes and plasma expanders. Zeitschrift für Zellforschung 45, 2–17 (1956). https://doi.org/10.1007/BF00320731
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DOI: https://doi.org/10.1007/BF00320731