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Monoclonal antibodies against T-cell antigens studied by immunohistochemistry

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Summary

Fifteen monoclonal antibodies against different T-cell antigens were studied by immunohistochemistry in thymus, fetal thymus, fetal liver, palatine tonsils, and a few T-cell lymphomas. OKT 9 was identified as reacting with hemopoietic stem or precursor cells in fetal liver as well as with early B-determined lymphocytes in tonsillar germinal centres. OKT 10 labelled lymphocytes in thymus and surprisingly also the cytoplasm of some tonsillar cells with plasma-cell like appearance. OKT 6 and MAS 036 b reacted only with thymic cells. OKT 4, OKT 5, OKT 8, 8–11, labelled thymic cells- and portions of interfollicular cells in tonsils. OKT 3, NEI 016, NEI 015, and T 28 stained a majority of thymic cells and of tonsillar interfollicular lymphocytes. IFH-M 203, NEI 012 and 4–11 were positive with the majority of T-lymphocytes in tonsils but labelled only a few thymic cells.

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Authors and Affiliations

  1. Institut für Hämatologie der GSF, Landwehrstraße 61, D-8000, München 2, Federal Republic of Germany

    G. Hoffmann-Fezer, I. Doxiadis, K. Stünkel, U. Kummer, M. Eulitz & S. Thierfelder

  2. Institut für Immunologie der Universität München, D-8000, München, Federal Republic of Germany

    J. Lohmeyer & E. P. Rieber

Authors
  1. G. Hoffmann-Fezer
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  2. J. Lohmeyer
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  3. I. Doxiadis
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  4. K. Stünkel
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  5. E. P. Rieber
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  6. U. Kummer
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  7. M. Eulitz
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  8. S. Thierfelder
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Supported by DFG (Ho 779/1) and SFB 37

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Hoffmann-Fezer, G., Lohmeyer, J., Doxiadis, I. et al. Monoclonal antibodies against T-cell antigens studied by immunohistochemistry. Blut 44, 275–288 (1982). https://doi.org/10.1007/BF00320702

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  • DOI: https://doi.org/10.1007/BF00320702

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