Summary
Bone marrow aplasia was induced by fractionated whole body irradiation with 3000 R and restitution was started by autologous bone marrow transplantation. During the period of aplasia the amount of buffy coat biopterin clearly followed the decline of leukocytes and, vice versa, during reconstitution it largely paralleled their increase in number. The amount of red cell biopterin closely correlated with the number of reticulocytes rather than with the fairly constant values for hematocrit or of erythrocytic protein. Thereby it clearly followed the various periods of red cell recovery. The amount of cellular biopterin, its concentration with respect to cell number or to unit of protein and the percentage distribution of biopterin among the cell fractions are presented as characteristics of the activity of hemopoietic cell proliferation.
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Kokolis N, Ziegler I (1968) Wiedererscheinen von Tetrahydrobiopterin in der Regenerationsknospe von Triturus-Arten. Z Naturforsch 23b: 860
Fukushima K, Eto I, Mayumi T, Richter W, Goodson S, Shiota T (1975) Biosynthesis of pterins in mammalian systems. In: Pfleiderer W (ed) Chemistry and biology of pteridines. Walter de Gruyter, Berlin, p 247
Kokolis N, Ziegler I (1977) On the levels of phenylalanine, tyrosine and tetrahydrobiopterin in the blood of tumor-bearing organisms. Cancer Biochem Biophys 2: 79
Halpern R, Halpern BC, Stea B, Dunlap A, Conklin K, Clark B, Ashe H, Sperling L, Halpern IA, Hardy D, Smith RA (1977) Pterin-6-aldehyde, a cancer cell catabolite: identification and application in diagnosis and treatment of human cancer. Proc Natl Acad Sci USA 74: 587
Rokos H, Rokos K, Frisius H, Kirstaedter HI (1980) Altered urinary excretion of pteridines in neoplastic disease. Determination of biopterin, neopterin, xanthopterin and pterin. Clin Chim Acta 105: 275
Fliedner TM (1970) Collection, cryopreservation and transplantation of blood stem cells in the treatment of hemopoietic failure. In: Thierfelder S, Rott H, Kolb HJ (eds) Immunobiology of bone marrow transplantation. Springer, Berlin, Heidelberg, New York, p 53
Bodenberger U, Kolb HJ, Rieder I, Netzel B, Schaffer E, Kolb H, Thierfelder S (1980) Fractionated total body irradiation and autologous bone marrow transplantation in dogs: hemopoietic recovery after various marrow cell doses. Exp Hematol 8: 384
Baker H, Frank O, Bacchi CT, Hutner SH (1974) Biopterin concent of human and rat fluids and tissues determined protozoologically. Am J Clin Nutr 27: 1247
Leeming RJ, Blair JA, Melikian V, O'Gorman DJ (1976) Biopterin derivates in human body fluids and tissues. J Clin Pathol 29: 444
Fukushima T, Nixon JC (1980) Analysis of reduced forms of biopterin in biological tissues and fluids. Anal Biochem 102: 176
Ziegler I, Fink M, Wilmanns W (1982) Biopterin level in peripheral blood cells as a marker for hemopoietic cell proliferation during leukemia and Polycythemia vera. Blut 44: 231–240
Kolb HJ, Rieder I, Bodenberger U, Netzel B, Schaffer E, Kolb H, Thierfelder S (1979) Dose rate and dose fractionation studies in total body irradiation of dogs. Pathol Biol 27: 370
Baker H, Frank O, Shapiro A, Hutner SH (1980) Assay of unconjugated pteridines in biological fluids and tissues with Crithidia. In: McCormick DB, Wright LD (eds) Methods in enzymology, vol 66. Academic Press, New York, p 490
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal Biochem 72: 248
Ziegler I (1965) Pterine als Wirkstoffe und Pigmente. Ergeb Physiol Biol Chem Exp Pharmakol 56: 1
Stea B, Halpern RM, Halpern BC, Smith RA (1980) Quantitative determination of pterins in biological fluids by high-performance liquid chromatography. J Chromatogr 188: 363
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Ziegler, I., Kolb, H.J., Bodenberger, U. et al. Biopterin level in blood cells as a marker for hemopoietic cell proliferation during autologous bone marrow transplantation in beagle dogs. Blut 44, 261–270 (1982). https://doi.org/10.1007/BF00320700
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DOI: https://doi.org/10.1007/BF00320700