Summary
Chromosome studies were performed in 24 patients who underwent allogeneic bone marrow transplantation (BMT) for severe aplastic anaemia (8), chronic myeloid leukemia (5 in chronic, 2 in accelerated phase and 1 in lymphoid blast crisis), acute myeloid leukemia (6), acute lymphoblastic leukemia in relapse (1) and Hodgkin's disease (1). Donor-cell type engraftment was demonstrated in 21 patients: in all 17 sex-mismatched transplants and — as demonstrated by reconstitution with Ph-negative cell populations — in 4 CML patients with a sex-matched donor. Recipient-type mitoses were seen in the bone marrow of 5 cases (1 SAA, 3 CML, 1 AML) after transplantation. They were only observed on one occasion in patients with SAA (4 of 25 on day 33) and AML (44 of 50 on day 14). Despite the continued demonstration of some Ph-positive mitoses in 3 patients with CML up to day 28, 323 and 451 after BMT, respectively, all surviving CML patients are still in complete haematological and clinical remission. So far the significance of these cytogenetically abnormal persisting host cells remains unknown.
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Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C (1976) Proposals for the classification of the acute leukaemias. Br J Haematol 33: 451–458
Boyd CN, Ramberg RC, Thomas ED (1982) The incidence of recurrence of leukemia in donor cells after allogeneic bone marrow transplantation. Leuk Res 6: 833–837
Branch DR, Gallagher MT, Forman SJ, Winkler KJ, Petz LD, Blume KG (1982) Endogenous stem cell repopulation resulting in mixed hematopoietic chimerism following total body irradiation and marrow transplantation for acute leukemia. Transplantation 34: 226–228
Caspersson T, Zech L, Johannsen C (1970) Differential binding of alkylating fluorochromes in human chromosomes. Exp Cell Res 60: 315–319
Chaganti RSK, RatnerLM, MC-KCC Bone Marrow Transplantation Group (1980) Use of chromosomal heteromorphisms in the analysis of haematologic reconstitution following haematogenic tissue transplantation. Am J Hum Genet 32: 65 A, Abstr. 199
Elfenbein GJ, Brogaonkar DS, Bias WB, Burns WH, Saral R, Sensenbrenner LL, Tutschka PJ, Zaczek BS, Zander AR, Epstein RB, Rowley JD, Santos GW (1978) Cytogenetic evidence for recurrence of acute myelogenous leukemia after allogeneic bone marrow transplantation in donor hematopoietic cells. Blood 52: 627–636
Fialkow PS, Thomas ED, Bryant JI, Neiman PE (1971) Leukaemic transformation of engrafted human marrow cells in vivo. Lancet i: 251–255
Gahrton G, Zech L, Friberg K, Lundgren G, Möller E, Groth CG (1976) Chromosomal satellites as markers in human bone-marrow transplantation. Hereditas 84: 15–18
Gale RP (1979) Advances in treatment of acute myelogenous leukemia. N Engl J Med 300: 1189–1199
Goldman JM, Apperley JF, Marcus RE, Hows J, Gordon-Smith EC, Reid CD, Catovsky D, Galton DAG (1985) Bone marrow transplantation for patients in the chronic phase of chronic granulocytic leukaemia: Apparent low incidence of relapse within the first two years post-BMT. Exp Hematol (Suppl. 17) 13: 14–15
Gosset TC, Gale RP, Fleischmann H, Austin GE, Sparkes RS, Taylor CR, Chir B (1979) Immunoblastic sarcoma in donor cells after bone marrow transplantation. N Engl J Med 300: 904–907
Haas OA, Schwarzmeier JD, Nacheva E, Fischer P, Paietta E (1984) Investigations on karyotype evolution in patients with chronic myeloid leukemia. Blut 48: 33–43
Hinterberger W, Schmidmeier W, Hajek-Rosenmayr A, Höcker P, Volc-Platzer B, Grabner G, Kallinger W, Alth G, Kolbabek H, Laszkowicz A, Mauritz W, Graninger W, Gadner H, Lechner K, Fischer M, Stingl G, Schwarzinger I, Druml I, Deutsch E (1985) Allogeneic BMT for chronic myelocytic leukaemia (CML): Some Ph+ bone marrow cells may resist cyclophosphamide and TBI conditioning but are gradually deleted during donor-type haemopoietic reconstitution. Exp Hematol (Suppl. 17) 13: 39
ISCN (1978) An International System for Chromosome Nomenclature, Harnden DG, Klinger HP (eds) Karger, Switzerland
Knuutila S, Vuopio P, Elonen E, Siimes M, Kovanen R, Borgström GH, de la Chapelle A (1981) Culture of bone marrow reveals more cells with chromosomal abnormalities than the direct method in patients with hematological disorders. Blood 58: 369–375
Lawler SD, Baker MC, Harris H, Morgenstern GR (1984) Cytogenetic studies on recipients of allogeneic bone marrow using the sex chromosomes as markers of cellular origin. Br J Haematol 56: 431–443
Littlefield LG (1972) An abnormal cell-line in a patient with acute granulocytic leukemia: cytogenetic studies before and after an allogneic marrow transplant. Cancer 29: 1281–1286
Marmont A, Frassoni F, Bacigalupo A, Podestà M, Piaggio G, Van Lint MT, Caimo A, De Filippi S (1984) Recurrence of Ph1-positive leukemia in donor cells after marrow transplantation for chronic granulocytic leukemia. N Engl J Med 310: 903–906
Newburger PE, Latt SA, Pesando JM, Gustashaw K, Powers M, Chaganti RSK, O'Reilly RJ (1981) Leukemia relapse in donor cells after allogeneic bone-marrow transplantation. N Engl J Med 304: 712–714
O'Reilly RJ (1983) Allogeneic bone marrow transplantation: current status and future directions. Blood 62: 941–964
Reiffers J, Bernard P, David B, Broustet A (1985) Autografting for chronic myeloid leukemia in transformation. (Letter to the editor.) Am J Hematol 18: 105–106
Sandberg AA, Kaiser-MacCaw, Hecht B, Hecht F (1985) Nomenclature: The Philadelphia chromosome or Ph without superscript. Cancer Genet Cytogenet 14: 1
Schmitz N, Gödde-Salz E, Löffler H (1985) Cytogenetic studies on recipients of allogeneic bone marrow transplants after fractionated total body irradiation. Br J Haematol 60: 239–244
Schubach WH, Hackman R, Neiman PE, Miller G, Thomas ED (1982) A monoclonal immunoblastic sarcoma in donor cells bearing Epstein-Barr virus genomes following allogeneic marrow grafting for acute lymphoblastic leukemia. Blood 60: 180–187
Schweizer D (1980) Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromsomes. Cytogenet Cell Genet 27: 190–193
Singer JW, Keating A, Ramberg R, McGuffin R, Sanders JE, Sale G, Fialkow PJ, Thomas ED (1983) Long-term stable hematopoietic chimerism following marrow transplantation for acute lymphoblastic leukemia: a case report with in vitro marrow culture studies. Blood 62: 869–872
Song CW, Kim TH, Khan FM, Kersey JH, Levitt SH (1981) Radiobiological basis of total body irradiation with different dose rate and fractionation: repair capacity of hemopoietic cells. Int J Radiol Oncol Biol Phys 7: 1695–1701
Sparkes RS (1981) Cytogenetic analysis in human bone marrow transplantation. Cancer Genet Cytogenet 4: 345–352
Speck B, Cornu B, Jeannet M, Nissen C, Burri HP, Groff P, Nagel GA, Buckner CD (1976) Autologous marrow recovery following allogeneic marrow transplantation in a patient with severe aplastic anemia. Exp Hematol 4: 131–137
Storb R, Santos GW (1983) Application of bone marrow transplantation in leukemia and aplastic anemia. Clin Haematol 12: 721–737
Sullivan KM, Parkman R (1983) The pathophysiology and treatment of graft-versus-host disease. Clin Haematol 12: 775–789
Thomas ED, Bryant JI, Buckner CD, Clift RA, Fefer A, Johnson FL, Neiman P, Ramberg RE, Storb R (1972) Leukaemic transformation of engrafted human marrow cells in vivo. Lancet i: 1310–1313
Thomas ED, Storb R, Clift RA, Fefer A, Johnson FL, Neimaron PE, Lerner KG, Glucksberg H, Bucknot CD (1975) Bone marrow transplantation. N Engl J Med 292: 832–843, 895–902
Wang HC, Federoff S (1972) Banding in human chromosomes treated with trypsin. Nature New Biol 235: 52–53
Witherspoon RP, Schubach W, Neimann P, Martin P, Thomas ED (1985) Donor cell leukemia developing six years after marrow grafting for acute leukemia. Blood 65: 1172–1180
Workshop on chromsomes in patients submitted to allogeneic bone marrow transplantation. Proceedings of the 3 rd International Course of Cytogenetics in Oncology. Bologna, May 2–4, 1985
Zaccaria A, Rosti G, Testoni N, Ricci P, Guardigli C, Rizzi S, Cavo M, Barbieri E, Tura S (1985) Serial chromosome studies in transplanted Ph 1+ CML patients. Sporadic observation of Ph 1+ cells after BMT. Exp Hematol (Suppl. 17) 13: 16
Zaccaria A, Rosti G, Testoni N, Gahrton G, Tura S (1986) Chromosome studies in patients with Philadelphia chromosome positive chronic myeloid leukemia submitted to bone marrow transplantation — Results of an European cooperative study. Cancer Genet Cytogenet (submitted for publication)
Zaccaria A, Rosti G, Testoni N, Frassoni F, Gahrton G, Tura S (1986) Chromosome studies in patients with acute non-lymphocytic leukemia and acute lymphocytic leukemia submitted to bone marrow transplantation — Results of an European cooperative study. Cancer Genet Cytogenet (submitted for publication)
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Present address: Roswell Park Memorial Institute, Department of Genetics and Endocrinology, 666 Elm Street, Buffalo, NY 14 222, USA
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Haas, O.A., Hinterberger, W., Schmidmeier, W. et al. Cytogenetic studies in bone marrow transplant recipients. Blut 53, 29–38 (1986). https://doi.org/10.1007/BF00320580
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DOI: https://doi.org/10.1007/BF00320580