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A monoclonal antibody to the heavy chain of human IgM with a particular reaction pattern in human B-cell malignancies

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Summary

We produced a monoclonal antibody to the human μ-chain. Its specificity was proved by aminoacid-sequence analysis of immunoabsorbent purified cell surface antigen. In binding studies an affinity constant (K-aff) of 5.7×109 M−1 was determined. When compared with a polyclonal anti-IgM antiserum it was found that this antibody may react with a determinant of the μ-chain differently exposed in clonal B-cell disorders rendering it to a reagent of particular diagnostic interest.

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Authors and Affiliations

  1. Department of Hematology and Bone Marrow Transplantation, National Medical Center and the Beckman Research Institute of the City of Hope, 1500 E. Duarte Road, 91010, Duarte, California, USA

    M. Henke & S. Santos

  2. James Irvine Center for the Study of Leukemias and Lymphomas, Department of Anatomic Pathology, National Medical Center and the Beckman Research Institute of the City of Hope, 1500 E. Duarte Road, 91010, Duarte, California, USA

    C. D. Winberg

  3. Division of Immunology, City of Hope, National Medical Center and the Beckman Research Institute of the City of Hope, 1500 E. Duarte Road, 91010, Duarte, California, USA

    J. E. Shively

Authors
  1. M. Henke
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  2. S. Santos
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  3. C. D. Winberg
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  4. J. E. Shively
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Additional information

Dedicated to Professor Dr. Dr. h. c. G. W. Löhr on his 6th birthday

M. Henke was the recipient of grant He 1286-1/1+2 from the Deutsche Forschungsgemeinschaft. Supported in part by grants CA 26422 and CA 09308 from the National Cancer Institute, by grant AI 19198 from the National Institute of Health, and by DHEW and Hematopathology Tutorials, Inc.

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Henke, M., Santos, S., Winberg, C.D. et al. A monoclonal antibody to the heavy chain of human IgM with a particular reaction pattern in human B-cell malignancies. Blut 55, 173–179 (1987). https://doi.org/10.1007/BF00320573

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  • DOI: https://doi.org/10.1007/BF00320573

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