Summary
The production of a monoclonal antibody against amyloid fibril protein AA, AAmc1, has been described. AAmc1, an IgG 2a,κ immunoglobulin, binds to isolated protein AA and in tissue sections to amyloid of only the AA-type. AAmc1 could, therefore, become a standard reagent for the diagnosis and in investigations on the pathogenesis of AA-amyloidoses.
This is a preview of subscription content, log in via an institution to check access.
References
Glenner GG (1980) Amyloid deposits and amyloidosis. The β-fibrilloses. N Engl J Med 302: 1283, 1333
Livini N, Laufer A, Levo Y (1980) Demonstration of amyloid in murine and human secondary amyloidosis by immunoperoxidase technique. J Pathol 132: 343
Linke RP, Nathrath WBJ (1980) Klassifizierung von Amyloid-Krankheiten an der Biopsie. Anwendung der Immunperoxidase-Technik. Münch Med Wochenschr 122: 1772
Fujihara S, Balow JE, Costa JC, Glenner GG (1980) Identification and classification of amyloid in formalin-fixed, paraffin-embedded tissue sections by the unlabelled immunoperoxidase method. Lab Invest 43: 358
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495
Linke RP, Heilmann KL, Nathrath WBJ, Eulitz M (1982) (submitted) Identification of amyloid-A protein in a sporadic Muckle-Wells syndrome. N-terminal amino acid sequence after isolation from formalin-fixed tissue
Linke RP (1980) Immunochemical investigations on serum amyloid A (SAA). Temperature-induced changes of its quarternary structure. In: Glenner GG, Costa PP, Freitas F (eds) Amyloid and amyloidosis. Excerpta Medica, Amsterdam, p 313
Linke RP, Sipe JD, Pollock PS, Ignaczak TF, Glenner GG (1975) Isolation of a low-molecular weight serum component antigenically related to an amyloid fibril protein of unknown origin. Proc Natl Acad Sci USA 72: 1473
McAdam KPWJ, Li J, Kuowles J, Foss NT, Dinarello CA, Rosenwasser LJ, Selinger MJ, Kaplan MM, Goodman R (1982) The biology of SAA. Identification of the inducer, in vitro synthesis, and heterogeneity demonstrated with monoclonal antibodies. Ann NY Acad Sci 389: 126
Author information
Authors and Affiliations
Additional information
Supported by Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg (Li 247/5-4)
Rights and permissions
About this article
Cite this article
Linke, R.P. Identification of amyloid protein AA with a monoclonal antibody. Blut 45, 407–409 (1982). https://doi.org/10.1007/BF00320551
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00320551