Summary
The aim of this study was to investigate the prognostic significance of density distribution profiles in non-Hodgkin lymphomas. Density profiles were evaluated by equilibrium centrifugation on linear polysucrose-metrizoat gradients.
Biopsy cells from 44 untreated non-Hodgkin lymphomas in advanced stages according to their density patterns could be classified into one of two distinct groups: Group A lymphomas exhibited high to intermediate densities and resembled normal resting B or T lymphocytes; they showed low spontaneous proliferation and favourable histologies. Group B lymphomas exhibited light densities, resembling activated lymphocytes and frequently revealed high spontaneous proliferation and unfavourable histology. Within a minimal time of observation of 40 months only 28% of the group A but 75% of the group B lymphomas died. Simultaneously performed surface marker studies excluded the possibility that variable proportions of normal lymphocytes contaminating the lymphoma suspensions are responsible for the two density groups. They rather suggested that these patterns reflect the individual capacity of a lymphoma to differentiate into denser tumour clones with low spontaneous proliferation. We therefore conclude that analyses of density profiles from non-Hodgkin lymphomas represents a promising method for the prognostic classification of this disease.
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Literatur
Bremer K, Meusers P, Brittinger G (1980) Chemotherapie der non-Hodgkin Lymphome. Internist 21: 512
Huber Ch, Dworzak E, Fink U, Michlmayr G, Huber H, Braunsteiner H (1974) Receptor sites for aggregated gammaglobulin (AGG) on the surface of normal lymphocytes and lymphocytes in lymphoproliferative disorders. Br J Haematol 27: 643
Huber Ch, Flad HD, Nilsson K, Wigzell H, Michlmayr G, Frischauf H, Braunsteiner H (1978) Expression and specifity of FcIgG-receptors on neoplastic lymphocytes. Blood 52: 911
Huber Ch, Huber H, Schmalzl F, Braunsteiner H (1971) Decreases proliferative activity of erythroblasts in granulocytic stem cell leukaemia. Nature (Lond) 229: 113
Huber Ch, Lutz D, Niederwieser D, Weiser G, Theml H, Braunsteiner H (1982) Prognostic significance of lymphocyte density distribution profiles in adult non-Hodgkin lymphomas. Blood (im Druck)
Huber Ch, Niederwieser D, Zier K, Braunsteiner H (1982) Dichteverteilung menschlicher Lymphozyten: I. Untersuchungsergebnisse an normalen lymphatischen Geweben. Blut 45: 375–383
Huber Ch, Zier K, Michlmayr G, Rodt H, Nilsson K, Theml H, Lutz O, Braunsteiner H (1978) A comparative study of the buoyant density distribution of normal and malignant lymphocytes. Br J Haematol 40: 93
Lennert K, Mohri N, Stein H, Kaiserling E (1975) The histopathology of malignant lymphoma. Br J Haematol 31 [Suppl 193]
Musshof K (1980) Strahlentherapie der non-Hodgkin Lymphome. Internist 21: 502
Silvestrini R, Piazza R, Riccardi A, Rilke F (1977) Correlation of cell kinetic findings with morphology of non Hodgkin's malignant lymphomas. J Natl Cancer Inst 58: 499
Zier KS, Huber Ch, Braunsteiner H (1977) Linear density gradient separation of human lymphocyte subsets. I. Analysis by mixed lymphocyte culture and cell mediated lympholysis responses. Eur J Immunol 7: 366
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Mit Unterstützung der Österreichischen Fonds »Zur Förderung der Wissenschaftlichen Forschung« No. 3174 und dem Jubiläumsfonds der Österreichischen Nationalbank
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Niederwieser, D., Huber, C., Lutz, D. et al. Dichteverteilung menschlicher Lymphozyten. Blut 45, 385–394 (1982). https://doi.org/10.1007/BF00320549
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DOI: https://doi.org/10.1007/BF00320549